| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP23B |
| Uniprot No | O75900 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 79-254aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSYTLTPARLRWDHFNLTYRILSFPRNLL SPRETRRALAAAFRMWSDVSPFSFREVAPEQPSDLRIGFYPINHTDCLVS ALHHCFDGPTGELAHAFFPPHGGIHFDDSEYWVLGPTRYSWKKGVWLTDL VHVAAHEIGHALGLMHSQHGRALMHLNATLRGWKALSQDELWGLHRLYG |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP23B重组蛋白的3篇参考文献摘要，供参考：
1. **文献名称**：*Expression and Characterization of Recombinant Human MMP23B in Insect Cells*
**作者**：Li Y et al.
**摘要**：该研究利用杆状病毒-昆虫细胞系统表达重组人MMP23B蛋白，并通过亲和层析纯化获得高纯度蛋白。功能分析表明，重组MMP23B具有金属蛋白酶活性，并能特异性降解明胶底物，提示其在细胞外基质重塑中的潜在作用。
2. **文献名称**：*Structural Insights into MMP23B: A Unique Member of the Matrix Metalloproteinase Family*
**作者**：Smith JR, et al.
**摘要**：通过X射线晶体学解析了重组MMP23B的晶体结构，发现其催化结构域与其他MMP家族成员差异显著。研究揭示了MMP23B缺乏典型的前肽结构域，可能通过非经典途径调控其酶活性，为靶向药物设计提供依据。
3. **文献名称**：*MMP23B Recombinant Protein Suppresses Tumor Cell Invasion via TIMP-3 Interaction*
**作者**：Chen H, et al.
**摘要**：体外实验表明，重组MMP23B通过结合组织抑制因子TIMP-3抑制肿瘤细胞迁移和侵袭。研究提示MMP23B可能通过竞争性结合TIMP调控其他MMP的活性，在癌症治疗中具有潜在应用价值。
注：以上文献信息为模拟示例，实际研究中请通过PubMed等数据库检索具体文献。若需真实文献，建议使用关键词"MMP23B recombinant protein"进行学术检索。
**Background of MMP23B Recombinant Protein**
MMP23B (Matrix Metalloproteinase-23B) is a member of the matrix metalloproteinase (MMP) family, which comprises zinc-dependent endopeptidases involved in extracellular matrix (ECM) remodeling, tissue repair, and cell signaling. Unlike most MMPs, MMP23B exhibits unique structural features, including the absence of a hinge region and a distinct C-terminal domain composed of an immunoglobulin-like (Ig-like) motif and a transmembrane segment. This atypical structure suggests specialized roles in cellular processes.
The MMP23B gene is located on chromosome 1p36 in humans, adjacent to its paralog MMP23A. It is expressed in reproductive tissues, such as the ovary and prostate, and has been implicated in cancer progression, inflammation, and developmental regulation. Studies highlight its potential involvement in ovarian carcinoma, prostate cancer, and immune responses, though its exact substrates and mechanisms remain less characterized compared to classical MMPs.
Recombinant MMP23B protein is engineered using expression systems like *E. coli* or mammalian cells to enable functional studies. Purification often involves affinity tags (e.g., His-tag) for high yield and specificity. This recombinant tool facilitates research into MMP23B's enzymatic activity, substrate specificity, and interactions with inhibitors or ECM components. Its role in modulating cytokines, chemokines, and ECM degradation underscores its relevance in pathological conditions, positioning it as a potential diagnostic marker or therapeutic target.
Current research focuses on clarifying MMP23B's dual roles in tissue homeostasis and disease, particularly its balance between protective and pathogenic functions. Further exploration may unlock insights into MMP-related therapies and disease mechanisms.
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