| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2I |
| Uniprot No | P36954 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKCG HKEAVFFQSH SARAEDAMRL YYVCTAPHCG HRWTE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2I重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "Structural and functional analysis of the human RNA polymerase II subunit RPB9"
**作者**: Cramer P, et al.
**摘要**: 该研究解析了人源RNA聚合酶II亚基RPB9(POLR2I编码)的重组蛋白晶体结构，揭示其与转录延伸复合物的相互作用，并证明其在转录保真性中的关键作用。
2. **文献名称**: "Recombinant expression and characterization of POLR2I in cancer cell proliferation"
**作者**: Smith JL, et al.
**摘要**: 通过大肠杆菌系统表达并纯化POLR2I重组蛋白，研究发现其过表达可抑制多种癌细胞增殖，提示其在肿瘤治疗中的潜在应用价值。
3. **文献名称**: "The role of POLR2I in transcriptional regulation: Insights from in vitro reconstitution assays"
**作者**: Tanaka K, et al.
**摘要**: 利用重组POLR2I蛋白进行体外转录重建实验，证明该亚基通过调控RNA聚合酶II的停顿和终止机制影响基因特异性转录效率。
注：上述文献信息为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议结合关键词“POLR2I recombinant protein”或“RNA polymerase II subunit RPB9”进一步查阅最新研究。
POLR2I, also known as RPB9. is a subunit of RNA polymerase II (Pol II), the enzyme responsible for synthesizing messenger RNA in eukaryotes. Encoded by the POLR2I gene, this protein is part of the core Pol II complex, which consists of 12 subunits in humans. POLR2I plays a critical role in transcription initiation and elongation. Structurally, it contains a conserved N-terminal zinc-binding domain and a C-terminal region that interacts with other Pol II components, contributing to the enzyme’s stability and catalytic activity.
Functionally, POLR2I is implicated in transcription fidelity and promoter selection. Studies suggest it assists in stabilizing the transcription pre-initiation complex and may influence the transition from initiation to elongation by modulating Pol II phosphorylation states. Additionally, POLR2I has been linked to transcription-coupled DNA repair mechanisms, highlighting its role in maintaining genomic integrity.
Recombinant POLR2I protein is engineered for in vitro studies to dissect Pol II assembly, transcriptional regulation, and interactions with transcription factors or regulatory complexes. Its recombinant form is typically expressed in bacterial or mammalian systems, often fused with tags (e.g., His-tag) for purification and detection. Researchers utilize this protein in structural studies (e.g., cryo-EM), biochemical assays, and drug discovery targeting transcription-related diseases.
Mutations or dysregulation of POLR2I have been associated with developmental disorders and cancers, underscoring its biomedical relevance. By studying recombinant POLR2I, scientists aim to unravel Pol II dysfunction mechanisms and develop therapeutic strategies for transcription-linked pathologies.
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