| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AASS |
| Uniprot No | Q9UDR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-926aa |
| 氨基酸序列 | MLQVHRTGLGRLGVSLSKGLHHKAVLAVRREDVNAWERRAPLAPKHIKGITNLGYKVLIQPSNRRAIHDKDYVKAGGILQEDISEACLILGVKRPPEEKLMSRKTYAFFSHTIKAQEANMGLLDEILKQEIRLIDYEKMVDHRGVRVVAFGQWAGVAGMINILHGMGLRLLALGHHTPFMHIGMAHNYRNSSQAVQAVRDAGYEISLGLMPKSIGPLTFVFTGTGNVSKGAQAIFNELPCEYVEPHELKEVSQTGDLRKVYGTVLSRHHHLVRKTDAVYDPAEYDKHPERYISRFNTDIAPYTTCLINGIYWEQNTPRLLTRQDAQSLLAPGKFSPAGVEGCPALPHKLVAICDISADTGGSIEFMTECTTIEHPFCMYDADQHIIHDSVEGSGILMCSIDNLPAQLPIEATECFGDMLYPYVEEMILSDATQPLESQNFSPVVRDAVITSNGTLPDKYKYIQTLRESRERAQSLSMGTRRKVLVLGSGYISEPVLEYLSRDGNIEITVGSDMKNQIEQLGKKYNINPVSMDICKQEEKLGFLVAKQDLVISLLPYVLHPLVAKACITNKVNMVTASYITPALKELEKSVEDAGITIIGELGLDPGLDHMLAMETIDKAKEVGATIESYISYCGGLPAPEHSNNPLRYKFSWSPVGVLMNVMQSATYLLDGKVVNVAGGISFLDAVTSMDFFPGLNLEGYPNRDSTKYAEIYGISSAHTLLRGTLRYKGYMKALNGFVKLGLINREALPAFRPEANPLTWKQLLCDLVGISPSSEHDVLKEAVLKKLGGDNTQLEAAEWLGLLGDEQVPQAESILDALSKHLVMKLSYGPEEKDMIVMRDSFGIRHPSGHLEHKTIDLVAYGDINGFSAMAKTVGLPTAMAAKMLLDGEIGAKGLMGPFSKEIYGPILERIKAEGIIYTTQSTIKP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AASS(α-氨基己二酸半醛合成酶)重组蛋白研究的示例参考文献(注：文献为假设示例，建议通过学术数据库查询真实研究)：
1. **文献名称**：*Expression and Purification of Recombinant Human AASS for Functional Analysis*
**作者**：Smith J, et al.
**摘要**：研究报道了人源AASS蛋白在大肠杆菌中的重组表达及纯化方法，验证了其催化赖氨酸代谢的酶活性，为研究AASS相关代谢疾病提供了实验基础。
2. **文献名称**：*Structural Insights into AASS Enzyme Mechanism via Recombinant Protein Crystallography*
**作者**：Chen L, et al.
**摘要**：通过重组表达并纯化AASS蛋白，解析其晶体结构，揭示了其催化赖氨酸转化为α-氨基己二酸半醛的分子机制。
3. **文献名称**：*AASS Deficiency: Recombinant Mutant Protein Analysis in Hyperlysinemia*
**作者**：Garcia R, et al.
**摘要**：构建了AASS基因突变体的重组蛋白，发现特定突变导致酶活性丧失，解释了高赖氨酸血症患者的代谢异常机制。
4. **文献名称**：*Optimization of AASS Recombinant Production in Mammalian Cells for Drug Screening*
**作者**：Wang Y, et al.
**摘要**：优化了哺乳动物细胞中AASS重组蛋白的表达体系，并应用于高通量药物筛选，探索潜在代谢疾病治疗策略。
建议通过 **PubMed/Google Scholar** 检索关键词“AASS recombinant protein”或“α-aminoadipic semialdehyde synthase”获取真实文献。
AASS (aminoadipic semialdehyde synthase) is a bifunctional enzyme encoded by the AASS gene, playing a critical role in the lysine degradation pathway. It catalyzes two sequential reactions: the conversion of lysine to saccharopine (lysine-ketoglutarate reductase activity) and subsequently to α-aminoadipic semialdehyde (saccharopine dehydrogenase activity). This metabolic pathway is essential in mammals for maintaining lysine homeostasis and producing intermediates linked to energy metabolism. Dysregulation of AASS has been associated with genetic disorders such as hyperlysinemia, characterized by elevated lysine levels and neurological complications.
Recombinant AASS protein is produced using biotechnological platforms, often through heterologous expression in bacterial (e.g., E. coli) or eukaryotic systems (e.g., insect or mammalian cells). The recombinant form enables detailed structural and functional studies, overcoming challenges in purifying the native enzyme from biological tissues. Researchers employ techniques like affinity chromatography and tag-based purification to obtain high-purity protein for biochemical assays, crystallography, or drug discovery. Its production has advanced understanding of lysine metabolism disorders and facilitated therapeutic exploration, including enzyme replacement strategies or inhibitor development.
Moreover, AASS recombinant protein serves as a tool to investigate substrate specificity, kinetic properties, and potential cross-talk with other metabolic pathways. Recent studies explore its role beyond lysine catabolism, such as in mitochondrial function and oxidative stress responses. The availability of recombinant AASS continues to support both basic research and translational applications in metabolic disease research.
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