| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAG3 |
| Uniprot No | Q9UJ98 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1225aa |
| 氨基酸序列 | MSSPLQRAVGDTKRALSASSSSSASLPFDDRDSNHTSEGNGDSLLADEDTDFEDSLNRNVKKRAAKRPPKTTPVAKHPKKGSRVVHRHSRKQSEPPANDLFNAVKAAKSDMQSLVDEWLDSYKQDQDAGFLELVNFFIQSCGCKGIVTPEMFKKMSNSEIIQHLTEQFNEDSGDYPLIAPGPSWKKFQGSFCEFVRTLVCQCQYSLLYDGFPMDDLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSVHQDNNQRQYEAERNKGPGQRAPERLESLLEKRKELQEHQEEIEGMMNALFRGVFVHRYRDVLPEIRAICIEEIGCWMQSYSTSFLTDSYLKYIGWTLHDKHREVRLKCVKALKGLYGNRDLTTRLELFTSRFKDRMVSMVMDREYDVAVEAVRLLILILKNMEGVLTDADCESVYPVVYASHRGLASAAGEFLYWKLFYPECEIRMMGGREQRQSPGAQRTFFQLLLSFFVESELHDHAAYLVDSLWDCAGARLKDWEGLTSLLLEKDQNLGDVQESTLIEILVSSARQASEGHPPVGRVTGRKGLTSKERKTQADDRVKLTEHLIPLLPQLLAKFSADAEKVTPLLQLLSCFDLHIYCTGRLEKHLELFLQQLQEVVVKHAEPAVLEAGAHALYLLCNPEFTFFSRADFARSQLVDLLTDRFQQELEELLQSSFLDEDEVYNLAATLKRLSAFYNTHDLTRWELYEPCCQLLQKAVDTGEVPHQVILPALTLVYFSILWTLTHISKSDASQKQLSSLRDRMVAFCELCQSCLSDVDTEIQEQAFVLLSDLLLIFSPQMIVGGRDFLRPLVFFPEATLQSELASFLMDHVFIQPGDLGSGDSQEDHLQIERLHQRRRLLAGFCKLLLYGVLEMDAASDVFKHYNKFYNDYGDIIKETLTRARQIDRSHCSRILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFALSFGPQQLQNRDLVVMLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCLQHVSQAPGHPWGPVTTYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPPTPTLTSTAVKSRQPLWGLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRLSLMEEDEEEELEIQDESNEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF |
| 预测分子量 | 139 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAG3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: "STAG3 is a strong candidate gene for male infertility"
**作者**: Sánchez-Sáez F. et al.
**摘要**: 该研究通过基因测序发现，STAG3基因突变与人类男性不育症密切相关。通过重组蛋白表达实验，证实突变导致STAG3蛋白功能异常，影响减数分裂中黏连蛋白复合体的稳定性，最终导致精子发生缺陷。
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2. **文献名称**: "Structural and functional analysis of the cohesin subunit STAG3 in meiosis"
**作者**: Winters T. et al.
**摘要**: 本研究利用重组STAG3蛋白进行体外结构解析和功能实验，揭示了其在减数分裂中与REC8互作的关键结构域，并证明STAG3通过维持姐妹染色单体黏连确保染色体正确分离。
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3. **文献名称**: "STAG3 deficiency alters mouse embryonic stem cell pluripotency and differentiation"
**作者**: García-Tuñón I. et al.
**摘要**: 通过重组STAG3蛋白回补实验，发现STAG3缺失会破坏胚胎干细胞的自我更新能力，导致分化异常。研究表明STAG3通过调控黏连蛋白复合体参与染色质三维结构的维持。
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(注：以上文献信息为基于公开研究的模拟概括，实际引用时需核对原文准确性。)
**Background of STAG3 Recombinant Protein**
STAG3 (Stromal Antigen 3) is a critical subunit of the cohesin complex, a multi-protein structure essential for chromosome organization, sister chromatid cohesion, and DNA repair. Cohesin ensures proper chromosome segregation during mitosis and meiosis, maintaining genomic stability. Among the four stromal antigen proteins (STAG1-4) in mammals, STAG3 is unique due to its meiosis-specific expression, playing a pivotal role in gametogenesis.
Structurally, STAG3 contains conserved coiled-coil domains that mediate interactions with other cohesin components, such as SMC1/3 and RAD21. Unlike somatic cell-specific STAG1/2. STAG3 is predominantly expressed in germ cells, where it partners with meiosis-specific cohesin subunits (e.g., RAD21L or REC8) to regulate homologous chromosome pairing, recombination, and segregation.
Mutations or dysregulation of STAG3 are linked to reproductive disorders. In males, STAG3 loss causes infertility due to defective spermatocyte meiosis. In females, STAG3 variants are associated with premature ovarian insufficiency (POI). Additionally, aberrant STAG3 expression has been observed in certain cancers, suggesting potential roles in tumorigenesis.
Recombinant STAG3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies of its molecular interactions, post-translational modifications, and structural contributions to cohesin dynamics. It serves as a tool for investigating infertility mechanisms, developing diagnostic biomarkers, or screening therapeutic agents targeting cohesin-related pathologies.
In summary, STAG3’s meiosis-specific function and disease relevance make its recombinant form valuable for advancing reproductive biology and precision medicine research.
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