| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | spi |
| Uniprot No | P17947 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 氨基酸序列 | MLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH |
| 预测分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条与 **SPI(沙门氏菌致病岛)重组蛋白** 相关的模拟参考文献示例(内容基于典型研究方向总结，非真实文献)：
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1. **文献名称**：*Functional characterization of SPI-1 effector SipA recombinant protein in Salmonella invasion*
**作者**：Garcia-Del Portillo, F. et al.
**摘要**：研究利用重组技术表达沙门氏菌SPI-1毒力岛中的SipA蛋白，揭示其通过调控宿主细胞肌动蛋白骨架促进细菌入侵的分子机制。
2. **文献名称**：*Recombinant SseB protein from SPI-2: A potential vaccine candidate against Salmonella Typhimurium*
**作者**：Hautefort, I. et al.
**摘要**：构建并纯化SPI-2分泌系统关键蛋白SseB的重组形式，通过动物实验验证其诱导保护性免疫应答的能力，为疫苗开发提供依据。
3. **文献名称**：*Structural analysis of SPI-4-encoded recombinant protein BapA in biofilm formation*
**作者**：Lambert, M.A. et al.
**摘要**：解析SPI-4编码的BapA重组蛋白的晶体结构，阐明其作为生物膜形成关键因子的功能域及作用机制。
4. **文献名称**：*Development of a diagnostic kit based on SPI-5 recombinant proteins for Salmonella detection*
**作者**：Marcus, S.L. et al.
**摘要**：基于SPI-5毒力岛重组蛋白(如SopB)的抗原性开发高特异性ELISA检测方法，显著提高临床样本中沙门氏菌的检出效率。
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**说明**：
- 以上文献为示例性内容，实际研究中建议通过 **PubMed/Web of Science** 等平台检索真实文献(关键词：*Salmonella SPI recombinant protein*)。
- SPI重组蛋白研究多聚焦于毒力因子功能、疫苗开发或诊断应用。
**Background of SPI Recombinant Proteins**
Recombinant proteins derived from *Salmonella* pathogenicity island 1 (SPI-1) represent a critical area of study in bacterial pathogenesis and host-pathogen interactions. SPI-1 is a 40 kb genomic region in *Salmonella enterica* that encodes a type III secretion system (T3SS), a molecular syringe-like apparatus used to inject effector proteins into host cells. These effectors manipulate host signaling pathways, facilitating bacterial invasion, immune evasion, and intracellular survival. SPI-1 is primarily associated with the early stages of infection, enabling *Salmonella* to breach intestinal epithelial barriers.
The development of SPI-1 recombinant proteins leverages genetic engineering to express and purify individual SPI-1-encoded proteins (e.g., SipB, SipC, InvA) in heterologous systems like *E. coli*. These proteins are studied to dissect their structural and functional roles in infection. For example, SipB and SipC are involved in membrane pore formation and actin cytoskeleton remodeling, respectively. Recombinant SPI-1 proteins also serve as tools for vaccine development, as they can elicit immune responses targeting key virulence factors.
Beyond basic research, SPI recombinant proteins have applications in drug discovery, enabling high-throughput screening for inhibitors of T3SS assembly or effector function. They are also used in diagnostic assays to detect *Salmonella*-specific antibodies in clinical or agricultural settings. However, challenges remain in maintaining the native conformation and post-translational modifications of these proteins during recombinant production, which can affect their biological activity.
Overall, SPI recombinant proteins bridge molecular microbiology and biotechnology, offering insights into bacterial virulence mechanisms while providing practical tools for therapeutic and diagnostic innovation. Their study continues to inform strategies for combating *Salmonella* infections and related enteric diseases.
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