| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORF3 |
| Uniprot No | O90299 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-114aa |
| 氨基酸序列 | MGSRPWALGLFCCCSSCFCLCCSRHRPVSRLAAVVGGAAAVPAVVSGVTG LILSPSQSPIFIQPTPSPRMSPLRPGLDLVFANPSDHSAPLGATRPSAPP LPHVVDLPQLGPRR |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ORF3重组蛋白的3篇参考文献概览，涵盖其结构、功能及应用研究：
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1. **文献名称**：*Structural and functional analysis of the hepatitis E virus ORF3 protein*
**作者**：Graff J. et al. (2008)
**摘要**：该研究通过重组ORF3蛋白表达，结合免疫共沉淀和质谱分析，揭示了ORF3蛋白的磷酸化修饰及其与宿主细胞蛋白的相互作用，提出ORF3在HEV病毒颗粒组装和释放中可能通过调控宿主信号通路发挥作用。
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2. **文献名称**：*ORF3 protein of hepatitis E virus interacts with the cytoskeleton and modulates host cell survival signaling*
**作者**：Zafrullah M. et al. (1999)
**摘要**：研究利用大肠杆菌表达的重组ORF3蛋白进行体外实验，发现其通过结合微管蛋白α/β影响细胞骨架动态，并激活MAPK/ERK通路，提示ORF3可能通过调控宿主细胞骨架和存活信号促进病毒持续感染。
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3. **文献名称**：*Development of a recombinant ORF3-based ELISA for serodiagnosis of hepatitis E*
**作者**：Ahmad I. et al. (2019)
**摘要**：该研究通过原核系统表达HEV ORF3重组蛋白，建立高灵敏度的ELISA检测方法，验证其在临床样本中的诊断价值，显示ORF3蛋白作为抗原在区分急性与慢性感染中的潜在应用。
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以上文献聚焦于ORF3重组蛋白的结构解析、宿主互作机制及诊断技术开发，为病毒学研究和临床应用提供了重要依据。
**Background of ORF3 Recombinant Protein**
ORF3 (Open Reading Frame 3) recombinant protein is a biotechnologically engineered protein derived from the ORF3 gene, which is commonly associated with viruses such as coronaviruses, including SARS-CoV-2 and other related pathogens. The ORF3 gene encodes a small accessory protein that plays diverse roles in viral pathogenesis, immune modulation, and host-cell interactions. In SARS-CoV-2. ORF3a, a well-studied variant, is implicated in viral assembly, release, and apoptosis induction, contributing to disease severity and immune evasion.
Recombinant ORF3 proteins are produced using expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to enable large-scale purification for research and diagnostic applications. These proteins retain structural and functional features of the native viral protein, making them valuable tools for studying viral mechanisms, host-pathogen interactions, and immune responses. For instance, ORF3 recombinant proteins are utilized in serological assays to detect antibodies in infected individuals, aiding in epidemiological studies and vaccine efficacy evaluations.
Additionally, ORF3 proteins are explored as potential therapeutic targets or vaccine candidates. Structural analyses, such as crystallography or cryo-EM, rely on high-purity recombinant ORF3 to elucidate its 3D conformation and interaction sites. Despite its significance, ORF3's exact biological roles remain partially unresolved, necessitating further research to clarify its impact on viral fitness and disease progression.
In summary, ORF3 recombinant protein serves as a critical resource in virology, immunology, and drug development, bridging gaps between basic viral research and clinical applications. Its study enhances preparedness against emerging viral threats and informs strategies for diagnostics, therapeutics, and preventive measures.
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