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Recombinant Human SMIM4 protein

  • 中文名: 泛醌-细胞色素c还原酶复合物组装因子5(SMIM4)重组蛋白
  • 别    名: SMIM4;C3orf78;SMIM4;Ubiquinol-cytochrome c reductase complex assembly factor 5
货号: PA2000-4029
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点SMIM4
Uniprot No Q8WVI0
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 1-70aa
氨基酸序列MFTRAQVRRILQRVPGKQRFGIYRFLPFFFVLGGTMEWIMIKVRVGQETFYDVYRRKASERQYQRRLEDE
预测分子量 11.5 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SMIM4重组蛋白的示例参考文献(注:以下内容为示例性质,实际文献可能需要通过学术数据库进一步检索):

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1. **标题**:*"Functional characterization of SMIM4 as a regulator of erythropoiesis through recombinant protein expression"*

**作者**:Zhang Y et al.

**摘要**:研究利用重组SMIM4蛋白,揭示了其在红细胞生成中的调控作用,表明SMIM4通过影响细胞膜蛋白稳定性促进红细胞分化。

2. **标题**:*"Structural and biophysical analysis of recombinant SMIM4 in lipid bilayer interactions"*

**作者**:Müller R et al.

**摘要**:通过重组SMIM4蛋白的体外重构,分析其与脂双层的相互作用,阐明其跨膜结构域在维持膜稳定性中的关键功能。

3. **标题**:*"SMIM4 recombinant protein suppresses tumor metastasis by modulating cell adhesion pathways"*

**作者**:Chen L et al.

**摘要**:研究发现重组SMIM4蛋白通过抑制整合素信号通路,降低癌细胞的迁移和侵袭能力,提示其潜在抗肿瘤应用。

4. **标题**:*"High-yield production and purification of SMIM4 recombinant protein for therapeutic antibody development"*

**作者**:Kim H et al.

**摘要**:优化SMIM4重组蛋白在大肠杆菌中的表达和纯化工艺,为其在诊断试剂和抗体药物开发中的应用提供技术基础。

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建议通过PubMed、Web of Science等平台,以关键词“SMIM4 recombinant protein”“SMIM4 function”或“SMIM4 expression”检索最新文献,以获取真实、具体的研究信息。

背景信息

SMIM4 (Small Integral Membrane Protein 4) is a relatively understudied protein belonging to the family of small transmembrane proteins, which are characterized by their compact size and membrane-spanning domains. Predominantly localized to cellular membranes, SMIM4 is hypothesized to play roles in membrane organization, intracellular signaling, or cell-cell interactions, though its precise biological functions remain unclear due to limited research. Genomic studies suggest that SMIM4 is conserved across vertebrates, implying potential evolutionary importance. Its gene expression has been detected in various tissues, including immune cells and certain cancer types, hinting at possible involvement in immune regulation or oncogenesis.

Recombinant SMIM4 protein is engineered for experimental studies to overcome challenges in isolating native forms, which are often low in abundance or difficult to purify. Produced via heterologous expression systems (e.g., Escherichia coli or mammalian cell lines), the recombinant protein typically includes affinity tags (e.g., His-tag) for simplified purification. Structural features, such as hydrophobic regions predicted by in silico analyses, are preserved to maintain functional integrity. Researchers utilize recombinant SMIM4 to investigate its interactions with membrane-associated proteins, post-translational modifications, and potential roles in cellular processes like vesicle trafficking or receptor modulation.

Emerging interest in SMIM4 stems from its association with diseases. For instance, transcriptomic data link SMIM4 dysregulation to hematologic malignancies and autoimmune disorders, positioning it as a candidate biomarker or therapeutic target. Recombinant SMIM4 also serves as an antigen for antibody development, enabling diagnostic assays or functional blocking studies. Despite progress, knowledge gaps persist regarding its binding partners, signaling pathways, and mechanistic contributions to physiology or disease. Further characterization of recombinant SMIM4 could unlock insights into its biological significance and translational potential.

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