| WB | 1/500 - 1/2000 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/100 - 1/500 | Human,Mouse,Rat,Rabbit |
| ICC | 技术咨询 | Human,Mouse,Rat,Rabbit |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Aliases | HKII; HXK2; DKFZp686M1669; HK2 |
| Entrez GeneID | 3099 |
| clone | 3D3 |
| WB Predicted band size | 102kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human HK2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HK2(己糖激酶2)抗体的3-4篇参考文献示例，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*Hexokinase 2 is a potential therapeutic target for glioblastoma*
**作者**：Wolf A, Agnihotri S, Micallef J, et al.
**摘要**：该研究通过免疫组化(使用HK2抗体)分析了HK2在胶质瘤组织中的高表达，发现其与患者预后不良相关，并探讨了靶向HK2抑制肿瘤代谢的潜在治疗策略。
2. **文献名称**：*Targeting Metabolic Reprogramming by HK2 Inhibition in Cancer*
**作者**：Roberts DJ, Miyamoto S.
**摘要**：综述文章总结了HK2在肿瘤代谢重编程中的核心作用，强调了HK2抗体在检测肿瘤组织特异性表达中的应用，并讨论了HK2作为癌症治疗靶点的分子机制及临床前研究进展。
3. **文献名称**：*Protocol for HK2-Specific Immunohistochemical Staining in Solid Tumors*
**作者**：Kwee SA, Tsujino T, Basu S.
**摘要**：该文章详细描述了使用HK2抗体进行组织样本免疫组化染色的标准化流程，验证了抗体在肝癌、乳腺癌等多种实体瘤中的特异性及敏感性。
4. **文献名称**：*HK2-Driven Glycolysis Promotes Hepatocellular Carcinoma Metastasis*
**作者**：Andersen JB, Riehle KJ, Park YN, et al.
**摘要**：研究通过HK2抗体检测肝癌组织中HK2的表达水平，发现HK2介导的糖酵解增强与肿瘤转移和患者生存率降低显著相关，提示靶向HK2可能抑制肝癌进展。
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以上文献均聚焦于HK2抗体在基础研究或临床检测中的应用，涵盖肿瘤代谢、预后评估及实验技术开发等领域。实际引用时建议核对期刊名称及作者全名以符合规范。
The HK2 antibody targets hexokinase 2 (HK2), a key enzyme in glycolysis that catalyzes the first step of glucose metabolism by converting glucose to glucose-6-phosphate. HK2 is primarily localized to the outer mitochondrial membrane, where it couples glycolysis to mitochondrial ATP production, enhancing energy efficiency. Unlike other hexokinase isoforms (e.g., HK1), HK2 is highly expressed in metabolically active tissues and is frequently overexpressed in cancer cells. This overexpression supports the Warburg effect, a metabolic hallmark of cancer characterized by increased glycolysis even under aerobic conditions. HK2 also interacts with mitochondrial voltage-dependent anion channels (VDACs), promoting tumor cell survival by inhibiting apoptosis.
The HK2 antibody is widely used in research to study metabolic reprogramming in cancer, neurodegenerative diseases, and diabetes. It enables detection of HK2 expression levels via techniques like Western blot, immunohistochemistry, and immunofluorescence. Studies using HK2 antibodies have revealed its role in tumor progression, chemoresistance, and as a potential therapeutic target. Inhibitors targeting HK2-mitochondria interactions are under exploration to disrupt cancer metabolism. However, HK2's dual role in normal and pathological metabolism necessitates careful evaluation of therapeutic strategies. Overall, the HK2 antibody serves as a critical tool for understanding metabolic dysregulation and developing targeted therapies.
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