| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBQLN1 |
| Uniprot No | Q9UMX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-589aa |
| 氨基酸序列 | AESGESGGPPGSQDSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS |
| 预测分子量 | 69.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBQLN1重组蛋白的3篇参考文献及其摘要概括：
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1. **"UBQLN1 mediates proteasome substrate targeting by interacting with ubiquitin chains through its UBA domain"**
*Kaye F.J., et al.*
该研究阐明了UBQLN1通过其泛素相关结构域(UBA)结合泛素链，并作为分子桥梁将泛素化底物传递给蛋白酶体进行降解的机制，揭示了其在泛素-蛋白酶体系统中的关键作用。
2. **"Structural insights into the ubiquitin recognition by UBQLN1 in autophagy regulation"**
*Lim J., et al.*
通过重组UBQLN1蛋白的晶体结构分析，揭示了其N端UBA和C端STI1结构域协同识别泛素链的模式，并提出UBQLN1通过介导自噬受体与错误折叠蛋白的互作调控选择性自噬。
3. **"UBQLN1 mutations linked to neurodegeneration impair chaperone-mediated protein clearance"**
*Almeida B., et al.*
研究发现与肌萎缩侧索硬化症(ALS)相关的UBQLN1突变体(如P497H)破坏重组蛋白的寡聚化能力，导致其无法有效传递错误折叠蛋白至自噬-溶酶体途径，加剧神经元毒性蛋白聚集。
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以上研究均涉及UBQLN1重组蛋白的功能验证、结构解析或疾病关联机制，为理解其在蛋白质质量控制中的作用提供重要依据。
UBQLN1 (ubiquilin-1) is a multifunctional adaptor protein belonging to the ubiquitin-like protein family, primarily involved in regulating protein degradation pathways. It plays a critical role in the ubiquitin-proteasome system by linking ubiquitinated substrates to the proteasome for degradation. Structurally, UBQLN1 contains an N-terminal ubiquitin-like (UBL) domain that interacts with the 26S proteasome and a C-terminal ubiquitin-associated (UBA) domain that binds polyubiquitin chains on misfolded or damaged proteins. This dual-domain architecture enables UBQLN1 to act as a shuttle, recognizing ubiquitinated cargo and delivering it to degradation machinery.
Recombinant UBQLN1 protein is produced through genetic engineering, often expressed in bacterial (e.g., E. coli) or mammalian systems for research applications. It is commonly purified using affinity tags (e.g., His-tag) for studies on protein-protein interactions, substrate recognition, and proteasomal regulation. Researchers utilize recombinant UBQLN1 to investigate its role in cellular quality control, particularly in neurodegenerative diseases like Alzheimer's, Parkinson's, and ALS, where dysfunctional protein clearance contributes to pathological protein aggregation. Studies also explore its involvement in autophagy, DNA repair, and endoplasmic reticulum stress responses. Dysregulation of UBQLN1 expression or mutations in its coding sequence have been linked to disease progression, making it a potential therapeutic target. Its recombinant form serves as a vital tool for structural studies, inhibitor screening, and mechanistic insights into ubiquitin-dependent proteostasis.
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