| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CCNB; CCNB1 |
| Entrez GeneID | 891 |
| clone | 5G6 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human CCNB1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于CCNB1抗体的3篇参考文献的简要概括：
1. **"Cyclin B1 is a prognostic indicator and a therapeutic target in hepatocellular carcinoma"**
- **作者**: Ding, Q., et al. (2015)
- **摘要**: 该研究利用CCNB1抗体通过免疫组化和Western blot技术，发现CCNB1在肝癌组织中过表达，且与肿瘤转移和不良预后显著相关，提示其可作为潜在治疗靶点。
2. **"Mechanisms of cyclin B1 degradation during mitotic exit"**
- **作者**: Porter, L.A., et al. (2003)
- **摘要**: 研究通过CCNB1抗体进行免疫沉淀和免疫荧光实验，揭示了泛素-蛋白酶体系统在细胞分裂后期调控CCNB1降解的具体机制，强调了其在细胞周期退出中的关键作用。
3. **"Validation of CCNB1 antibody specificity in diverse experimental models"**
- **作者**: Smith, J., et al. (2020)
- **摘要**: 文章系统评估了多种商业CCNB1抗体的特异性，通过敲除细胞系和交叉实验验证其可靠性，为后续研究提供了抗体选择的参考依据。
(注：以上为模拟示例，实际文献需通过数据库检索确认。)
The CCNB1 antibody targets cyclin B1. a key regulatory protein involved in cell cycle progression, particularly during the transition from the G2 phase to mitosis. Cyclin B1 forms a complex with cyclin-dependent kinase 1 (CDK1), which drives the initiation of mitosis by phosphorylating substrates essential for nuclear envelope breakdown, chromosome condensation, and spindle assembly. Dysregulation of CCNB1 expression is linked to uncontrolled cell proliferation and genomic instability, making it a biomarker of interest in cancer research. Overexpression of CCNB1 has been observed in various malignancies, including breast, lung, and colorectal cancers, and is often associated with poor prognosis, metastasis, and therapeutic resistance.
CCNB1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels, subcellular localization (nuclear vs. cytoplasmic), and cell cycle dynamics in both research and clinical settings. These antibodies aid in studying cell cycle checkpoints, drug responses, and mechanisms of oncogenesis. Commercially available CCNB1 antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated silencing. Researchers also utilize them to explore therapeutic strategies targeting the cyclin B1-CDK1 axis, such as small-molecule inhibitors, to restore cell cycle control in cancer. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary, allowing flexibility for experimental design.
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