| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPRR1A |
| Uniprot No | P35321 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-89aa |
| 氨基酸序列 | MNSQQQKQPCTPPPQPQQQQVKQPCQPPPQEPCIPKTKEPCHPKVPEPCHPKVPEPCQPKVPEPCQPKVPEPCPSTVTPAPAQQKTKQK |
| 预测分子量 | 17.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPRR1A重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"SPRR1A is a key component of the human keratinocyte damage response pathway"*
**作者**: Pastore S, et al.
**摘要**: 该研究揭示了SPRR1A在角质形成细胞应对紫外线(UVB)或炎症因子(如TNF-α)刺激中的核心作用。通过重组蛋白实验，发现SPRR1A表达上调可增强细胞抗氧化应激能力，并参与表皮屏障修复的调控。
2. **文献名称**: *"Proteolytic processing of the SPRR1A protein during keratinocyte differentiation"*
**作者**: Tarcsa E, et al.
**摘要**: 研究利用重组SPRR1A蛋白分析其在角质化包膜(cornified envelope)形成中的功能，发现其通过转谷氨酰胺酶介导的交联反应参与细胞分化，并证实其结构中的富脯氨酸区域对蛋白稳定性至关重要。
3. **文献名称**: *"SPRR1A as a potential biomarker in squamous cell carcinoma: Role of recombinant protein in vitro"*
**作者**: Kalinin AE, et al.
**摘要**: 通过重组SPRR1A蛋白的功能实验，发现其在鳞状细胞癌中异常高表达，可能与肿瘤侵袭性相关。研究提示SPRR1A可能通过调节细胞黏附通路影响癌症进展，为潜在治疗靶点提供依据。
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以上文献聚焦于SPRR1A重组蛋白在皮肤屏障、分化及肿瘤中的分子机制，涉及表达调控、结构功能及临床关联研究。
**Background of SPRR1A Recombinant Protein**
Small proline-rich protein 1A (SPRR1A) is a member of the SPRR family, a group of cysteine-rich, cross-linking proteins critical for epithelial barrier formation and terminal differentiation of keratinocytes. Encoded by the *SPRR1A* gene on human chromosome 1q21. SPRR1A is predominantly expressed in stratified squamous epithelia, such as skin, esophagus, and oral mucosa. It plays a key role in the assembly of the cornified cell envelope (CCE), a specialized structure that provides mechanical strength and protection against environmental stressors, pathogens, and dehydration. SPRR1A interacts with other CCE components, including loricrin, involucrin, and transglutaminases, through disulfide bonds and glutamine-lysine cross-links during epidermal maturation.
Beyond its structural role, SPRR1A is implicated in cellular stress responses, inflammation, and wound healing. It is upregulated by oxidative stress, UV radiation, and cytokines (e.g., IL-6. TNF-α), suggesting involvement in adaptive mechanisms to tissue damage. Dysregulation of SPRR1A has been linked to skin pathologies, such as psoriasis, atopic dermatitis, and squamous cell carcinoma, where abnormal keratinocyte differentiation or proliferation occurs.
Recombinant SPRR1A protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains bioactivity for functional studies. It is widely used to investigate epidermal differentiation pathways, protein-protein interactions, and therapeutic targets for skin disorders. Recent studies also explore its potential role in cancer progression, as SPRR1A may exhibit dual oncogenic and tumor-suppressive effects depending on context. Its recombinant form enables high-throughput screening for drug candidates and mechanistic insights into epithelial biology.
Overall, SPRR1A recombinant protein serves as a vital tool for understanding epithelial homeostasis and developing treatments for dermatological and oncological diseases.
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