| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLQ |
| Uniprot No | O75417 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-987aa |
| 氨基酸序列 | MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFR |
| 预测分子量 | 112.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于POLQ(DNA聚合酶theta)重组蛋白的关键参考文献及摘要概括：
1. **"POLQ (Pol theta), a DNA polymerase and DNA helicase, mediates resistance to ionizing radiation and cisplatin"**
- 作者: Yoshimura, M. et al.
- 摘要：研究发现POLQ在哺乳动物细胞中通过易错的DNA修复途径(如微同源介导的末端连接)参与DNA损伤修复，其高表达与对电离辐射和顺铂的耐药性相关，提示POLQ可作为癌症治疗的潜在靶点。
2. **"DNA polymerase theta protects against genome instability by promoting replication restart"**
- 作者: Ceccaldi, R. et al.
- 摘要：POLQ通过重启停滞的复制叉来维持基因组稳定性，尤其在BRCA缺陷的癌细胞中，POLQ的缺失导致复制叉崩溃和染色体异常，揭示了其在复制压力应答中的关键作用。
3. **"Targeting DNA polymerase theta (POLQ) for synthetic lethality in BRCA-deficient cancers"**
- 作者: Higgins, G.S. et al.
- 摘要：该研究证实POLQ抑制剂与PARP抑制剂在BRCA突变肿瘤中表现出合成致死效应，为BRCA1/2缺失的癌症患者提供了新的联合治疗策略，并解析了POLQ在双链断裂修复中的独特机制。
4. **"Structural basis of DNA synthesis by polymerase theta during DNA repair"**
- 作者: Newman, J.A. et al.
- 摘要：通过冷冻电镜解析POLQ-DNA复合物结构，揭示其独特的跨病变合成能力和末端结合域对微同源配对的促进作用，为设计特异性抑制剂提供了结构基础。
POLQ (DNA polymerase theta) is a specialized DNA polymerase involved in DNA repair and genome maintenance, primarily through the theta-mediated end-joining (TMEJ) pathway, a backup double-strand break (DSB) repair mechanism. Unlike canonical repair pathways like homologous recombination (HR) or non-homologous end-joining (NHEJ), TMEJ operates when primary repair mechanisms fail, often utilizing microhomology to bridge DNA ends. POLQ’s unique structure—a helicase-like domain fused to a polymerase domain—enables both strand displacement and synthesis activities, making it critical for resolving complex DNA lesions, including those induced by radiation or chemotherapeutics.
Recombinant POLQ proteins are engineered for in vitro studies to dissect its enzymatic functions, structural features, and interactions. Produced via heterologous expression systems (e.g., insect or bacterial cells), these purified proteins retain key biochemical properties, allowing researchers to probe TMEJ mechanisms, polymerase fidelity, and helicase activity. Recent studies highlight POLQ’s dual role in cancer: its overexpression in certain tumors correlates with genomic instability and poor prognosis, yet it also represents a therapeutic target. Inhibitors targeting POLQ’s polymerase or helicase domains are under investigation to sensitize cancer cells to DNA-damaging therapies.
Recombinant POLQ has further applications in synthetic biology and genome-editing tool development, leveraging its ability to process damaged or mismatched DNA. However, its error-prone nature poses challenges, necessitating precise characterization. Ongoing research aims to unravel POLQ’s role in immune diversification, replication stress response, and its potential as a biomarker for therapy resistance. Overall, recombinant POLQ serves as a vital tool for understanding genome resilience and advancing targeted anticancer strategies.
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