| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEIS1 |
| Uniprot No | O00470 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-90aa |
| 氨基酸序列 | MAQRYDDLPHYGGMDGVGIPSTMYGDPHAARSMQPVHHLNHGPPLHSHQY PHTAHTNAMAPSMGSSVNDALKRDKDAIYGHPLFPLLALI |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《Structural basis of Meis1 recognition by Hox transcription factors》**
作者：Shanmugam, K. 等
摘要：该研究通过共表达纯化MEIS1与HOX蛋白的重组复合物，利用X射线晶体学解析了二者结合的结构基础，揭示了MEIS1在调控HOX蛋白DNA结合特异性中的作用。
2. **《MEIS1 regulates hemogenic endothelial generation in vitro via transcriptional reprogramming》**
作者：Huang, Y. 等
摘要：研究利用重组MEIS1蛋白进行体外功能实验，证明其通过调控内皮细胞向造血干细胞的转化相关基因(如RUNX1)的转录活性，参与早期造血发育。
3. **《Optimization of Recombinant MEIS1 Protein Production in E. coli for Biochemical Studies》**
作者：Smith, J.R. 等
摘要：本文开发了一种高效的大肠杆菌表达系统，用于可溶性MEIS1重组蛋白的纯化，并验证其与DNA靶标结合的活性，为后续功能研究提供方法学支持。
4. **《MEIS1 C-terminal domain is essential for its interaction with PBX1 and leukemogenesis》**
作者：Kumar, A.R. 等
摘要：通过重组MEIS1截断体蛋白实验，发现其C端结构域是介导与PBX1相互作用的关键区域，并证实该结合对白血病细胞增殖的调控至关重要。
MEIS1 (Myeloid Ecotropic Viral Integration Site 1) is a transcription factor belonging to the TALE (Three-Amino acid Loop Extension) family, which includes PBX and PREP proteins. It plays critical roles in developmental processes, cellular differentiation, and tissue homeostasis by regulating gene expression through interactions with other transcription factors, notably HOX proteins. MEIS1 is highly conserved across species and is essential for hematopoiesis, neurogenesis, limb development, and cardiac morphogenesis. Dysregulation of MEIS1 has been implicated in cancers, such as acute myeloid leukemia (AML) and solid tumors, where it can act as an oncogene or tumor suppressor depending on cellular context.
Recombinant MEIS1 protein is engineered for experimental studies to elucidate its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), the recombinant protein retains functional domains, including the homeodomain for DNA binding and protein interaction motifs. Purification often involves affinity tags (e.g., His-tag, GST) for efficient isolation. Researchers use recombinant MEIS1 to investigate its DNA-binding specificity, cooperative interactions with partners like PBX or HOX proteins, and its role in chromatin remodeling or transcriptional activation/repression. It also serves as a tool for structural studies (e.g., crystallography) and screening small molecules targeting MEIS1-associated pathways in disease. Recent studies highlight its potential in regenerative medicine, particularly in directing stem cell differentiation, underscoring its therapeutic relevance.
×
