| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MEKK2; MEKK2B |
| Entrez GeneID | 10746 |
| clone | 4B4 |
| WB Predicted band size | 70kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP3K2 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇关于MAP3K2抗体的参考文献摘要整理：
1. **"MAP3K2 regulates Ras/Raf/MEK/ERK signaling in colorectal cancer through interaction with CDC25"**
*作者：Zhang Y, et al.*
摘要：该研究利用MAP3K2抗体进行免疫共沉淀实验，揭示了MAP3K2通过CDC25调控结直肠癌中Ras/MAPK信号通路的机制，为靶向治疗提供新方向。
2. **"Antibody-based profiling of MAP3K2 in hepatocellular carcinoma reveals oncogenic kinase activation"**
*作者：Li H, et al.*
摘要：通过MAP3K2抗体的免疫组化分析，发现肝细胞癌中MAP3K2蛋白高表达且与患者预后不良相关，提示其作为生物标志物的潜力。
3. **"MAP3K2 controls immune response in T cells via JNK/c-Jun signaling"**
*作者：Wang Q, et al.*
摘要：研究使用MAP3K2抗体敲低蛋白表达，证明MAP3K2通过JNK通路调控T细胞免疫应答，为自身免疫疾病治疗提供理论依据。
4. **"Development of a phospho-specific MAP3K2 antibody for monitoring kinase activity in lung adenocarcinoma"**
*作者：Sato R, et al.*
摘要：该文献报道了一种磷酸化特异性MAP3K2抗体的开发，用于检测肺癌细胞中MAP3K2激酶活性，并验证其与EGFR信号通路的关联。
注：以上为模拟文献摘要，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“MAP3K2 antibody”、“MAP3K2 kinase signaling”结合研究领域(如癌症、炎症)查找近期论文。
The MAP3K2 (Mitogen-Activated Protein Kinase Kinase Kinase 2) antibody is a crucial tool for studying the MAPK signaling pathway, which regulates cellular responses to stress, growth factors, and cytokines. MAP3K2. a serine/threonine kinase, activates downstream MAP2Ks (e.g., MEK1/2) and MAPKs (e.g., ERK1/2), influencing processes like proliferation, differentiation, and apoptosis. Dysregulation of MAP3K2 is linked to cancer, inflammatory diseases, and developmental disorders, making its detection vital for mechanistic research.
Antibodies targeting MAP3K2 enable the analysis of its expression, post-translational modifications, and interactions in various experimental models (e.g., Western blot, immunohistochemistry, immunofluorescence). They are often validated for specificity using knockout cell lines or tissues to minimize cross-reactivity with homologous kinases like MAP3K3. Researchers use these antibodies to explore MAP3K2's role in tumorigenesis—particularly in RAS-driven cancers—and its interplay with scaffolding proteins that modulate pathway specificity. Additionally, MAP3K2 inhibitors are under investigation for therapeutic applications, heightening the demand for reliable antibodies to assess target engagement and efficacy in preclinical studies. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyoclonal) vary, allowing flexibility across applications. Proper validation ensures accurate interpretation of MAP3K2's spatiotemporal regulation and contribution to disease mechanisms.
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