| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIGLEC1 |
| Uniprot No | Q9BZZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1709aa |
| 氨基酸序列 | MGFLPKLLLLASFFPAGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFMAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVNHPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHANAARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLYPPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSLAAPISLHVSYAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQAVNVQVWPGATVREGQLVNLTCLVWTTHPAQLTYTWYQDGQQRLDAHSIPLPNVTVRDATSYRCGVGPPGRAPRLSRPITLDVLYAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEGVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQVLYAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRLQVEGARVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGSASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKQSMGENSVEMAFQKETTQLIDPDAATCETSTCAPPLG |
| 预测分子量 | 182,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIGLEC1重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**：Structural basis for recognition of lymphotropic virus by the innate immune inhibitor CD169/SIGLEC1
**作者**：Izquierdo-Usero N, et al.
**摘要**：该研究解析了SIGLEC1重组蛋白的晶体结构，发现其通过N端V-set结构域特异性结合HIV-1病毒颗粒表面的神经节苷脂GM3.揭示了其作为天然免疫抑制受体在病毒感染中的分子机制。
2. **文献名称**：SIGLEC1 (CD169) mediates antigen capture and immune activation in SARS-CoV-2 infection
**作者**：Zhou Y, et al.
**摘要**：研究利用重组SIGLEC1蛋白证明其通过结合新冠病毒刺突蛋白的唾液酸化结构域，促进树突状细胞对病毒颗粒的内吞和交叉呈递，为开发基于SIGLEC1的疫苗佐剂提供理论基础。
3. **文献名称**：Recombinant SIGLEC1-Fc fusion protein as a therapeutic candidate for autoimmune disorders
**作者**：Chen Q, et al.
**摘要**：该团队开发了SIGLEC1胞外段与人IgG1 Fc的重组融合蛋白，在类风湿性关节炎小鼠模型中显示出通过负向调控B细胞活化显著减轻炎症反应，提出其作为自身免疫疾病治疗的新策略。
注：以上文献为虚拟示例，实际引用需以真实发表论文为准。建议通过PubMed或Web of Science以"SIGLEC1 recombinant protein"为关键词检索最新研究。
Sialic acid-binding immunoglobulin-type lectin 1 (SIGLEC1. also known as CD169) is a transmembrane protein belonging to the SIGLEC family of cell surface receptors, which recognize sialic acid residues on glycoproteins and glycolipids. Primarily expressed on macrophages and dendritic cells, SIGLEC1 acts as a pattern recognition receptor involved in immune modulation. It binds sialylated pathogens, such as viruses and bacteria, facilitating their uptake for antigen presentation and promoting interactions with other immune cells, including T and B lymphocytes. This receptor plays dual roles in immunity, balancing pathogen clearance with the suppression of excessive inflammation to maintain homeostasis.
Structurally, SIGLEC1 contains an N-terminal V-set immunoglobulin (Ig) domain responsible for sialic acid binding, followed by multiple C2-set Ig domains and a transmembrane region with a cytoplasmic tail housing signaling motifs. Recombinant SIGLEC1 proteins are typically engineered as soluble forms (e.g., extracellular domains fused with Fc tags or affinity tags) using mammalian expression systems, preserving ligand-binding functionality while enabling experimental flexibility.
Research applications include studying viral entry mechanisms (e.g., HIV-1. SARS-CoV-2) that exploit SIGLEC1 for immune cell targeting, investigating tumor-associated immunosuppression through interactions with sialylated cancer cells, and exploring its role in autoimmune diseases like systemic lupus erythematosus (SLE). Recombinant SIGLEC1 also serves as a tool for developing therapeutic inhibitors or targeted drug delivery systems aimed at modulating immune responses. Its unique position at the intersection of pathogen recognition and immune regulation makes it a critical focus in infectious disease, oncology, and immunotherapy research.
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