| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSK3 |
| Uniprot No | P49841 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420aa |
| 氨基酸序列 | MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL PNGRDTPALF NFTTQELSSN PPLATILIPP HARIQAAAST PTNATAASDA NTGDRGQTNN AASASASNST HHHHHH |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GSK3重组蛋白的3篇参考文献及其摘要概括：
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1. **"Crystal structure of glycogen synthase kinase 3β: structural basis for phosphate-primed substrate specificity and autoinhibition"**
- **作者**: Dajani, R., et al. (2001)
- **摘要**: 本研究通过X射线晶体学解析了人源GSK3β重组蛋白的三维结构，揭示了其磷酸化依赖的底物识别机制及自抑制构象。研究发现ATP结合口袋的构象变化为设计选择性抑制剂提供了结构基础。
2. **"Expression and purification of active recombinant GSK3α/β in mammalian cells for high-throughput screening"**
- **作者**: Frame, S., et al. (2003)
- **摘要**: 文章描述了在哺乳动物细胞(HEK293)中高效表达并纯化具有催化活性的GSK3α和GSK3β重组蛋白的方法。纯化蛋白成功应用于高通量药物筛选，鉴定出多个ATP竞争性小分子抑制剂。
3. **"GSK3 recombinant protein as a tool to study tau hyperphosphorylation in Alzheimer's disease models"**
- **作者**: Hernandez, F., et al. (2009)
- **摘要**: 研究利用重组GSK3β蛋白在体外诱导Tau蛋白过度磷酸化，模拟阿尔茨海默病病理特征。实验表明GSK3抑制剂可显著降低Tau磷酸化水平，验证了其在神经退行性疾病中的治疗潜力。
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以上文献涵盖GSK3重组蛋白的结构解析、表达方法及疾病机制研究，均为该领域经典研究。如需更多方向(如药物开发)，可进一步补充。
Glycogen synthase kinase 3 (GSK3) is a highly conserved serine/threonine protein kinase originally identified for its role in glycogen metabolism regulation. It exists as two isoforms, GSK3α and GSK3β, encoded by distinct genes but sharing 85% sequence homology. Unlike most kinases, GSK3 is constitutively active under basal conditions and primarily regulated through inhibitory phosphorylation at specific residues (Ser21 in GSK3α and Ser9 in GSK3β) by upstream signals such as insulin-activated Akt. This unique regulation allows GSK3 to act as a critical node in diverse signaling pathways, including Wnt/β-catenin, Hedgehog, and growth factor cascades, influencing processes like cell proliferation, differentiation, and apoptosis.
Recombinant GSK3 proteins are engineered to enable precise biochemical and functional studies. Typically expressed in prokaryotic (E. coli) or eukaryotic (insect or mammalian) systems, these proteins retain catalytic activity and post-translational modification capabilities when produced in appropriate expression systems. Purification tags (e.g., GST, His-tag) facilitate isolation while preserving enzyme functionality. Researchers utilize recombinant GSK3 to investigate substrate interactions, screen small-molecule inhibitors (e.g., lithium, CHIR99021), and dissect pathway mechanisms in diseases. Notably, GSK3 dysregulation has been implicated in Alzheimer’s disease (via tau hyperphosphorylation), diabetes (insulin resistance), and cancer (modulating oncogenic transcription factors). The development of isoform-specific recombinant proteins has further advanced understanding of their distinct roles, as GSK3β knockout is embryonically lethal in mice, whereas GSK3α deletion shows metabolic phenotypes. By providing a controlled, high-purity enzyme source, recombinant GSK3 remains indispensable for both basic research and therapeutic discovery targeting this multifaceted kinase.
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