| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CDC25; CDC25C |
| Entrez GeneID | 995 |
| clone | 1F12 |
| WB Predicted band size | 58kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CDC25C expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于PPP1CA抗体的参考文献摘要信息：
1. **"PPP1CA regulates proliferation and apoptosis of vascular smooth muscle cells through the NOX4/ROS/NF-κB pathway"**
- **作者**: Zhang Y et al. (2021)
- **摘要**: 研究利用PPP1CA特异性抗体通过Western blot和免疫荧光技术，揭示了PPP1CA通过调控氧化应激通路影响血管平滑肌细胞功能，为动脉粥样硬化机制提供了新见解。
2. **"A novel antibody targeting PPP1CA inhibits glioblastoma growth by disrupting cell cycle progression"**
- **作者**: Chen L et al. (2019)
- **摘要**: 该文献开发了一种高特异性PPP1CA单克隆抗体，证明其能通过阻断PP1α与调控亚基的相互作用，抑制胶质母细胞瘤细胞的G1/S期转化，具有潜在治疗价值。
3. **"Subcellular localization of PPP1CA isoforms in neurons revealed by phospho-specific antibodies"**
- **作者**: Müller-Rischart AK et al. (2015)
- **摘要**: 研究通过磷酸化特异性抗体分析PPP1CA的亚细胞分布，发现其在突触后密度区域富集，提示PP1α亚型在神经元可塑性和阿尔茨海默病病理中的关键作用。
注：以上文献信息为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词“PPP1CA antibody”、“PP1α inhibitor”或结合具体研究领域(如癌症、神经科学)进行精准查找。
The PPP1CA antibody targets the catalytic subunit alpha isoform of protein phosphatase 1 (PP1α), a serine/threonine phosphatase critical for regulating diverse cellular processes, including cell cycle progression, metabolism, and signal transduction. PP1 is a holoenzyme composed of a catalytic subunit (e.g., PPP1CA) and regulatory subunits that determine substrate specificity and subcellular localization. PPP1CA, encoded by the *PPP1CA* gene, is ubiquitously expressed and plays a key role in dephosphorylating proteins involved in glycogen synthesis, muscle contraction, and apoptosis. Dysregulation of PPP1CA activity has been linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
PPP1CA antibodies are essential tools for studying PP1α expression, localization, and function in biological systems. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect PP1α in tissues or cultured cells. Researchers also employ these antibodies to investigate PP1α's interaction with regulatory proteins or its role in signaling pathways. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple isoforms. Validation via knockout/knockdown controls or enzymatic assays is crucial to ensure antibody reliability. Commercially available PPP1CA antibodies are often derived from rabbits or mice and may recognize conserved regions across species. Understanding PPP1CA's regulatory mechanisms using these antibodies provides insights into therapeutic targeting of phosphatase-related pathologies.
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