| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | plnA |
| Uniprot No | P80214 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-48aa |
| 氨基酸序列 | AYSLQMGATAIKQVKKLFKKWGW |
| 预测分子量 | 18.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **plnA重组蛋白** 的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **标题**: *"Heterologous Expression and Antimicrobial Characterization of plnA from Lactobacillus plantarum in E. coli"*
**作者**: Lee, S., et al.
**摘要**: 研究报道了植物乳杆菌plnA基因在大肠杆菌中的重组表达与纯化，证实重组蛋白对革兰氏阳性病原菌(如金黄色葡萄球菌)具有显著抑菌活性，并探讨了其作用机制。
2. **标题**: *"Structural Insights into plnA: Crystallographic Analysis of a Bacteriocin Regulatory Protein"*
**作者**: Smith, J.R., & García-Lara, J.
**摘要**: 通过X射线晶体学解析了plnA蛋白的三维结构，揭示了其与细菌素生物合成基因簇的相互作用，为理解plnA在群体感应调控中的功能提供结构基础。
3. **标题**: *"Functional Characterization of Recombinant plnA in Biofilm Inhibition of Foodborne Pathogens"*
**作者**: Zhang, Y., et al.
**摘要**: 研究评估了重组plnA蛋白对单核细胞增生李斯特菌等食源性病原菌生物膜形成的抑制作用，表明其在食品防腐中的应用潜力。
4. **标题**: *"Optimization of plnA Expression in Pichia pastoris for Enhanced Antimicrobial Peptide Production"*
**作者**: Müller, C., & Novak, J.
**摘要**: 利用毕赤酵母系统优化plnA重组蛋白的表达条件，显著提高产量，并验证其热稳定性和广谱抗菌活性。
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**注意**：以上文献为模拟示例，实际研究中请通过 **PubMed/Google Scholar** 检索关键词(如 "plnA recombinant protein"、"plantaricin plnA")获取真实文献。
**Background of plnA Recombinant Protein**
PlnA is a key regulatory protein associated with the production of plantaricin, a class II bacteriocin synthesized by *Lactobacillus plantarum*, a lactic acid bacterium widely found in fermented foods and the gastrointestinal tract. Bacteriocins are antimicrobial peptides that inhibit the growth of closely related bacterial strains, offering a competitive advantage in microbial ecosystems. PlnA plays a dual role: it acts as a transcription regulator for the plantaricin operon and participates in quorum sensing, a cell-density-dependent signaling mechanism that coordinates bacterial behavior.
The plnA gene is part of the *pln* locus, which includes structural and regulatory genes responsible for bacteriocin synthesis, secretion, and immunity. PlnA, along with PlnB, forms a two-component regulatory system that activates plantaricin production under specific environmental conditions, such as high cell density or nutrient stress. This regulation ensures energy-efficient antimicrobial activity, aligning bacteriocin synthesis with ecological needs.
Recombinant PlnA protein is engineered through heterologous expression systems, such as *E. coli*, to study its structure-function relationships and regulatory mechanisms. The protein’s small size (typically 50-60 amino acids) and helical structure facilitate its interaction with DNA and other regulatory proteins. Studies on PlnA have provided insights into bacteriocin biosynthesis, stress response pathways, and interspecies competition in microbial communities.
Applications of PlnA recombinant protein extend to food preservation, where plantaricin-producing strains or purified bacteriocins inhibit spoilage organisms and pathogens like *Listeria*. Additionally, its role in quorum sensing has sparked interest in developing anti-biofilm agents or microbiome-modulating therapies. Challenges remain in optimizing production yields, stability, and delivery systems for industrial or clinical use. Ongoing research aims to harness PlnA’s regulatory properties for sustainable antimicrobial strategies amid rising antibiotic resistance concerns.
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