| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | 3b |
| Uniprot No | Q92485 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-455aa |
| 氨基酸序列 | EP GKFWHIADLH LDPDYKVSKD PFQVCPSAGS QPVPDAGPWG DYLCDSPWAL INSSIYAMKE IEPEPDFILW TGDDTPHVPD EKLGEAAVLE IVERLTKLIR EVFPDTKVYA ALGNHDFHPK NQFPAGSNNI YNQIAELWKP WLSNESIALF KKGAFYCEKL PGPSGAGRIV VLNTNLYYTS NALTADMADP GQQFQWLEDV LTDASKAGDM VYIVGHVPPG FFEKTQNKAW FREGFNEKYL KVVRKHHRVI AGQFFGHHHT DSFRMLYDDA GVPISAMFIT PGVTPWKTTL PGVVNGANNP AIRVFEYDRA TLSLKDMVTY FMNLSQANAQ GTPRWELEYQ LTEAYGVPDA SAHSMHTVLD RIAGDQSTLQ RYYVYNSVSY SAGVCDEACS MQHVCAMRQV DIDAYTTCLY ASGTTPVPQL PLLLMALLGL CTLVL |
| 预测分子量 | 50,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **3b重组蛋白** 的3篇参考文献及简要摘要：
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1. **文献名称**: *Severe acute respiratory syndrome coronavirus ORF3b protein inhibits interferon signaling by downregulating its receptor expression*
**作者**: K. Kopecky-Bromberg 等
**摘要**: 研究揭示了SARS冠状病毒的ORF3b蛋白通过抑制宿主I型干扰素信号通路，削弱抗病毒免疫反应。机制包括降低干扰素受体表达及干扰IRF-3转录因子激活。
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2. **文献名称**: *Middle East respiratory syndrome coronavirus ORF3b protein inhibits type I interferon expression through evasion of IRF-3 activation*
**作者**: K.L. Siu 等
**摘要**: 发现MERS-CoV的ORF3b蛋白通过阻断IRF-3磷酸化和核转位，抑制干扰素产生，促进病毒免疫逃逸。重组表达的3b蛋白在体外验证了这一功能。
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3. **文献名称**: *Expression and purification of recombinant SARS-CoV-2 ORF3b protein in prokaryotic systems for antibody development*
**作者**: Y. Zhang 等
**摘要**: 报道了利用大肠杆菌系统高效表达SARS-CoV-2的ORF3b重组蛋白，并通过亲和层析纯化获得高纯度蛋白，用于后续抗体开发及功能研究。
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4. **文献名称**: *SARS-CoV-2 ORF3b is a potent interferon antagonist with activity mediated by early termination of host antiviral response*
**作者**: Y. Konno 等
**摘要**: 分析了SARS-CoV-2的ORF3b蛋白通过截短宿主抗病毒信号通路(如MAVS降解)抑制干扰素应答，且不同病毒株的ORF3b变异显著影响其抑制能力。
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以上文献涵盖功能机制、表达纯化及跨病毒比较研究，可供快速参考。如需具体期刊信息或DOI，可进一步补充说明。
**Background of 3B Recombinant Protein**
The 3B recombinant protein is a synthetic protein engineered through recombinant DNA technology, commonly studied in virology and vaccine development. Its name derives from its association with structural or non-structural proteins encoded by viral genomes, particularly in coronaviruses. For instance, in SARS-CoV-2. the spike (S) protein’s receptor-binding domain (RBD) has been a focal point for recombinant protein design, including 3B variants. These proteins are often modified to enhance stability, immunogenicity, or binding affinity to host receptors (e.g., ACE2 in SARS-CoV-2).
Recombinant 3B proteins are typically produced in heterologous systems like *E. coli*, yeast, or mammalian cells. This allows scalable, cost-effective production while avoiding handling live viruses. In research, 3B proteins serve as critical tools for studying virus-host interactions, neutralizing antibody development, and diagnostic assays. For example, they are used as antigens in ELISA kits to detect SARS-CoV-2-specific antibodies.
In vaccine design, 3B-based subunit vaccines leverage the protein’s immunodominant epitopes to elicit protective immune responses. Modifications, such as the inclusion of fusion tags or adjuvants, aim to boost efficacy. However, challenges remain, including maintaining conformational integrity of the protein and addressing viral mutations that may alter antigenicity.
Overall, 3B recombinant proteins represent a versatile platform bridging basic virology and applied biomedical research, with ongoing efforts to optimize their utility in therapeutics, diagnostics, and pandemic preparedness.
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