| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA3G |
| Uniprot No | Q9NS98 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-782aa |
| 氨基酸序列 | GPSPGPSVPRLRLSYRDLLSANRSAIFLGPQGSLNLQAMYLDEYRDRLFLGGLDALYSLRLDQAWPDPREVLWPPQPGQREECVRKGRDPLTECANFVRVLQPHNRTHLLACGTGAFQPTCALITVGHRGEHVLHLEPGSVESGRGRCPHEPSRPFASTFIDGELYTGLTADFLGREAMIFRSGGPRPALRSDSDQSLLHDPRFVMAARIPENSDQDNDKVYFFFSETVPSPDGGSNHVTVSRVGRVCVNDAGGQRVLVNKWSTFLKARLVCSVPGPGGAETHFDQLEDVFLLWPKAGKSLEVYALFSTVSAVFQGFAVCVYHMADIWEVFNGPFAHRDGPQHQWGPYGGKVPFPRPGVCPSKMTAQPGRPFGSTKDYPDEVLQFARAHPLMFWPVRPRHGRPVLVKTHLAQQLHQIVVDRVEAEDGTYDVIFLGTDSGSVLKVIALQAGGSAEPEEVVLEELQVFKVPTPITEMEISVKRQMLYVGSRLGVAQLRLHQCETYGTACAECCLARDPYCAWDGASCTHYRPSLGKRRFRRQDIRHGNPALQCLGQSQEEEAVGLVAATMVYGTEHNSTFLECLPKSPQAAVRWLLQRPGDEGPDQVKTDERVLHTERGLLFRRLSRFDAGTYTCTTLEHGFSQTVVRLALVVIVASQLDNLFPPEPKPEEPPARGGLASTPPKAWYKDILQLIGFANLPRVDEYCERVWCRGTTECSGCFRSRSRGKQARGKSWAGLELGKKMKSRVHAEHNRTPREVEAT |
| 预测分子量 | 89.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA3G重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：Semaphorin 3G regulates hematopoietic stem-cell niche retention and extramedullary hematopoiesis
**作者**：Liu C, et al.
**摘要**：研究利用重组SEMA3G蛋白探究其在造血干细胞微环境中的作用，发现其通过调控细胞迁移影响骨髓外造血，提示其在血液疾病中的潜在治疗价值。
2. **文献名称**：SEMA3G inhibits tumor angiogenesis by suppressing VEGF signaling in endothelial cells
**作者**：Zhang Y, et al.
**摘要**：通过表达重组SEMA3G蛋白，验证其抑制血管内皮细胞中VEGF信号通路的功能，为靶向抗血管生成治疗肿瘤提供了新思路。
3. **文献名称**：Structural characterization of recombinant human SEMA3G and its interaction with neuropilin receptors
**作者**：Wang L, et al.
**摘要**：报道重组人源SEMA3G蛋白的晶体结构解析，揭示其与神经纤毛蛋白受体结合的分子机制，为神经导向机制研究奠定结构基础。
(注：以上文献信息为模拟示例，实际引用需以具体发表内容为准。)
**Background of SEMA3G Recombinant Protein**
SEMA3G (Semaphorin-3G) is a secreted protein belonging to the class III semaphorin family, which plays critical roles in neural development, immune regulation, and angiogenesis. Semaphorins are characterized by a conserved ~500-amino acid Sema domain, followed by immunoglobulin-like (Ig) and basic C-terminal domains. SEMA3G is expressed in various tissues, including the brain, heart, and vascular endothelial cells, and functions as a guidance cue for axon pathfinding and vascular patterning by interacting with plexin and neuropilin receptors.
Structurally, recombinant SEMA3G is produced using expression systems (e.g., mammalian or prokaryotic cells) to ensure proper folding and post-translational modifications. Its recombinant form retains functional domains necessary for receptor binding and signaling, enabling studies on its role in modulating cell migration, adhesion, and apoptosis. Dysregulation of SEMA3G has been linked to pathologies such as cancer, neurodegenerative disorders, and cardiovascular diseases. For instance, SEMA3G exhibits tumor-suppressive properties by inhibiting angiogenesis and metastasis in certain cancers.
Research applications of SEMA3G recombinant protein include elucidating its signaling pathways, exploring therapeutic potential in regenerative medicine, and developing biomarkers for disease diagnosis. Its ability to modulate immune responses and neuronal repair also positions it as a candidate for treating inflammatory or neurodegenerative conditions. Challenges remain in understanding context-dependent functions, receptor specificity, and optimal delivery mechanisms. Overall, SEMA3G recombinant protein serves as a vital tool for decoding semaphorin biology and advancing translational research.
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