| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Wnt6 |
| Uniprot No | P22727 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-364aa |
| 氨基酸序列 | LWWAVGSPLVMDPTSICRKARRLAGRQAELCQAEPEVVAELARGARLGVRECQFQFRFRRWNCSSHSKAFGRVLQQDIRETAFVFAITAAGASHAVTQACSMGELLQCGCQAPRGRAPPRPSGLLGTPGPPGPTGSPDASAAWEWGGCGDDVDFGDEKSRLFMDAQHKRGRGDIRALVQLHNNEAGRLAVRSHTRTECKCHGLSGSCALRTCWQKLPPFREVGARLLERFHGASRVMGTNDGKALLPAVRTLKPPGRADLLYAADSPDFCAPNRRTGSPGTRGRACNSSAPDLSGCDLLCCGRGHRQESVQLEENCLCRFHWCCVVQCHRCRVRKELSLCL |
| 预测分子量 | 50.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Wnt6重组蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**: *Wnt6 regulates epithelial stem cell proliferation and hair follicle regeneration*
**作者**: Willert, K., et al.
**摘要**: 该研究利用哺乳动物细胞表达系统成功制备了具有生物活性的重组Wnt6蛋白，并发现其在皮肤上皮干细胞增殖和毛囊再生中起关键作用。实验表明，外源性添加Wnt6重组蛋白可激活β-catenin信号通路，促进损伤后组织修复。
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2. **文献名称**: *Wnt6-mediated signaling pathway in colorectal cancer progression*
**作者**: Saito-Diaz, K., et al.
**摘要**: 研究通过大肠癌细胞模型，探讨了重组Wnt6蛋白对肿瘤细胞迁移和侵袭的影响。结果显示，Wnt6通过非经典信号通路(如JNK/STAT3)调控肿瘤微环境，重组蛋白的应用显著增强了癌细胞的转移能力。
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3. **文献名称**: *Structural and functional characterization of recombinant Wnt6 in embryonic development*
**作者**: Nusse, R., et al.
**摘要**: 该文献通过昆虫细胞系统表达并纯化了重组Wnt6蛋白，解析其脂质修饰对蛋白稳定性和信号活性的重要性。体内实验表明，Wnt6重组蛋白在鸡胚胎模型中促进体节形成，揭示了其在胚胎发育中的保守功能。
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4. **文献名称**: *Recombinant Wnt proteins in tissue engineering: Insights from Wnt6*
**作者**: Katoh, M., et al.
**摘要**: 研究评估了重组Wnt6蛋白在组织工程中的应用潜力，发现其可协同其他生长因子(如BMP2)促进间充质干细胞向成骨细胞分化，为骨再生治疗提供了实验依据。
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**备注**：上述文献为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索。若需具体文献，建议使用关键词“Wnt6 recombinant protein”或“Wnt6 expression”进一步筛选。
Wnt6 recombinant protein is a engineered form of the Wnt6 signaling molecule, a member of the evolutionarily conserved Wnt family of secreted glycoproteins. Wnt proteins play critical roles in embryonic development, tissue homeostasis, and cellular communication through canonical (β-catenin-dependent) and non-canonical signaling pathways. Specifically, Wnt6 has been implicated in regulating embryonic patterning, epithelial-mesenchymal interactions, and organogenesis, particularly in skin development, limb formation, and neural crest cell migration. Unlike some Wnt proteins requiring lipid modification for activity, Wnt6 is classified as a non-lipidated Wnt family member, enabling more straightforward recombinant production in standard expression systems.
The recombinant Wnt6 protein is typically produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and solubility. Purification methods often employ affinity tags for efficient recovery while maintaining biological activity. Researchers utilize Wnt6 recombinant protein in vitro to study its role in stem cell maintenance, tissue regeneration, and cancer biology, where aberrant Wnt signaling is frequently observed. It has shown potential in organoid culture systems for maintaining epithelial progenitor cells and modulating cell fate decisions.
Current challenges in Wnt6 recombinant protein research include maintaining its stability due to inherent aggregation tendencies of Wnt proteins and achieving pathway-specific activation. Recent studies explore its therapeutic potential in regenerative medicine and targeted cancer therapies. Commercial availability of bioactive Wnt6 recombinant protein has accelerated mechanistic studies, though careful validation of batch consistency and signaling specificity remains essential for experimental reproducibility.
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