| 纯度 | >80 % Purified via His tag. |
| 种属 | Human |
| 靶点 | AKR1B10 |
| Uniprot No | O60218 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-316aa |
| 氨基酸序列 | MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFNAEY |
| 预测分子量 | 63.kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于AKR1B10重组蛋白的模拟参考文献(实际文献需根据具体研究查询)：
1. **文献名称**：Expression and purification of recombinant human AKR1B10 and its enzymatic characterization
**作者**：Zhang Y, et al.
**摘要**：本研究通过大肠杆菌表达系统成功克隆并纯化了人源AKR1B10重组蛋白，分析了其对多种羰基底物(如视黄醛、4-氧壬烯醛)的还原酶活性，证实其在脂质过氧化产物代谢中的作用。
2. **文献名称**：AKR1B10 overexpression promotes tumor progression and correlates with clinical prognosis in hepatocellular carcinoma
**作者**：Cao D, et al.
**摘要**：通过重组AKR1B10蛋白功能实验，发现其在肝癌细胞中通过调控视黄酸代谢和Wnt/β-catenin通路促进肿瘤增殖和转移，为肝癌治疗提供潜在靶点。
3. **文献名称**：Structural basis of AKR1B10 inhibitors discovered by virtual screening
**作者**：Wang L, et al.
**摘要**：利用重组AKR1B10蛋白进行晶体结构解析，结合虚拟筛选技术鉴定了新型小分子抑制剂，揭示了其与酶活性位点的相互作用机制，为抗癌药物开发奠定基础。
(注：以上为示例性内容，实际文献需通过PubMed或Web of Science等数据库检索。)
AKR1B10 (aldo-keto reductase family 1 member B10) is a NADPH-dependent enzyme belonging to the aldo-keto reductase superfamily. It catalyzes the reduction of carbonyl-containing compounds, including aldehydes, ketones, and xenobiotics, playing a role in detoxification, metabolic regulation, and lipid synthesis. Initially identified as an enzyme overexpressed in hepatocellular carcinoma, AKR1B10 has since been linked to various cancers, including lung, breast, and colorectal cancers, where its upregulation is associated with tumor progression, chemoresistance, and poor prognosis.
The recombinant AKR1B10 protein is produced via heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and pathological mechanisms. Structurally, it shares a conserved (α/β)8-barrel fold typical of AKR enzymes, with substrate specificity influenced by active-site residues. Research highlights its role in metabolizing chemotherapeutic agents (e.g., daunorubicin) and lipid-derived aldehydes like 4-hydroxynonenal, which are implicated in oxidative stress and carcinogenesis. Additionally, AKR1B10 regulates fatty acid synthesis by stabilizing acetyl-CoA carboxylase, linking it to cancer cell proliferation.
Recombinant AKR1B10 is widely used in drug discovery to screen inhibitors targeting its enzymatic activity, with potential therapeutic applications in cancer and diabetic complications. Its overexpression in tumors also positions it as a biomarker for early cancer detection. However, conflicting studies note tissue-specific roles, as AKR1B10 is downregulated in certain gastrointestinal cancers, suggesting context-dependent functions. Ongoing studies aim to clarify its dual roles in normal physiology and disease, leveraging recombinant protein tools to explore structure-function relationships and therapeutic targeting strategies.
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艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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