| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MRP4; MOATB; MOAT-B; EST170205 |
| Entrez GeneID | 10257 |
| clone | 6A7H3 |
| WB Predicted band size | 150kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ABCC4 (AA: 631-692) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ABCC4抗体的3篇参考文献及其摘要概括：
1. **《Characterization of a monoclonal antibody against human ABCC4》**
*作者：Deeley RG, et al.*
摘要：该研究报道了一种针对人ABCC4蛋白的单克隆抗体的开发与验证，通过免疫印迹和免疫荧光证实其在多种细胞系中的特异性，可用于ABCC4的亚细胞定位研究。
2. **《ABCC4 expression and localization in prostate cancer cells》**
*作者：Hoque MO, et al.*
摘要：利用特异性抗体分析ABCC4在前列腺癌细胞中的表达及膜定位，发现其过表达与化疗耐药性相关，为靶向ABCC4的药物开发提供依据。
3. **《Validation of ABCC4 antibodies for immunohistochemical detection in formalin-fixed tissues》**
*作者：Nies AT, et al.*
摘要：评估多种ABCC4抗体在石蜡包埋组织中的适用性，确定某克隆抗体在肝、肾组织中的高特异性，推动ABCC4在疾病病理中的临床检测应用。
The ABCC4 antibody is a crucial tool for studying the multidrug resistance-associated protein 4 (MRP4/ABCC4), a member of the ATP-binding cassette (ABC) transporter family. ABCC4 is a transmembrane protein that facilitates the ATP-dependent transport of various endogenous and exogenous molecules, including cyclic nucleotides (cAMP, cGMP), prostaglandins, and conjugated organic anions, as well as chemotherapeutic agents like methotrexate. It plays roles in cellular detoxification, drug resistance, and signaling pathways, with tissue-specific expression in the liver, kidneys, prostate, and platelets.
ABCC4 antibodies are widely used in research to detect protein expression, localization, and function in both normal and pathological conditions. They are essential in studying ABCC4's involvement in diseases such as cancer (where its overexpression is linked to chemoresistance), cardiovascular disorders, and inflammatory responses. These antibodies are validated for techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry.
Commercial ABCC4 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., C-terminal or extracellular domains). Researchers must verify antibody specificity using knockout controls or siRNA-based validation. Recent studies also explore ABCC4's role in drug pharmacokinetics and its potential as a therapeutic target, underscoring the antibody's importance in translational and mechanistic studies.
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