| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OC3 |
| Entrez GeneID | 390874 |
| clone | 10B2F5 |
| WB Predicted band size | 50kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ONECUT3 (AA: 350-455) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ONECUT3抗体的3篇参考文献(基于模拟数据整理，建议通过学术数据库验证实际文献)：
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1. **文献名称**：*ONECUT3 promotes pancreatic cancer metastasis through transcriptional regulation of EMT-related genes*
**作者**：Smith J, et al. (2020)
**摘要**：研究利用ONECUT3抗体进行免疫组化分析，发现ONECUT3在胰腺导管腺癌中高表达，且与肿瘤转移和患者预后不良相关。机制研究表明其通过调控上皮-间质转化(EMT)通路驱动侵袭。
2. **文献名称**：*Hepatocyte differentiation requires ONECUT3-mediated transcriptional networks*
**作者**：Lee H, et al. (2018)
**摘要**：通过ONECUT3特异性抗体进行染色质免疫沉淀(ChIP-seq)和Western blot，揭示了ONECUT3在肝细胞分化中调控关键基因的表达，并验证了其与代谢功能相关因子的相互作用。
3. **文献名称**：*ONECUT3 as a novel biomarker in neuroendocrine tumors: Diagnostic and therapeutic implications*
**作者**：Garcia R, et al. (2021)
**摘要**：研究使用ONECUT3抗体进行免疫荧光和流式细胞术检测，证实其在神经内分泌肿瘤中特异性高表达，提示其可作为潜在诊断标志物或治疗靶点。
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**注意**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台以关键词“ONECUT3 antibody”或“HNF6γ antibody”检索确认。
The ONECUT3 antibody is a research tool targeting the ONECUT3 protein, a member of the ONECUT (also known as HNF6) family of transcription factors. ONECUT3. encoded by the *ONECUT3* gene in humans, is characterized by a single cut domain and a divergent homeobox domain, enabling sequence-specific DNA binding and regulation of gene expression. This protein plays critical roles in embryonic development, particularly in the specification and differentiation of tissues such as the liver, pancreas, and nervous system. It is involved in maintaining cellular identity and function, with studies linking it to glucose metabolism, hormone production, and neuronal development.
Antibodies against ONECUT3 are widely used in molecular and cell biology to investigate its expression, localization, and interactions. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in the study of ONECUT3’s role in health and disease. Dysregulation of ONECUT3 has been implicated in pathologies, including cancers (e.g., hepatocellular carcinoma, prostate cancer) and metabolic disorders. Research using these antibodies helps uncover mechanisms underlying tumor progression, metastasis, and metabolic dysregulation, offering potential therapeutic targets. Validation of ONECUT3 antibodies ensures specificity, minimizing cross-reactivity with related family members like ONECUT1 or ONECUT2. which share structural similarities but exhibit distinct functional roles. Overall, ONECUT3 antibodies are vital for advancing understanding of developmental biology and disease pathogenesis.
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