| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOSR2 |
| Uniprot No | O14653 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDPLFQQ THKQVHEIQS CMGRLETADK QSVHIVENEI QASIDQIFSR LERLEILSSK EPPNKRQNAR LRVDQLKYDV QHLQTALRNF QHRRHAREQQ ERQREELLSR TFTTNDSDTT IPMDESLQFN SSLQKVHNGM DDLILDGHNI LDGLRTQRLT LKGTQKKILD IANMLGLSNT VMRLIEKRAF QDK |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GOSR2重组蛋白的3篇参考文献，按文献发表时间排序：
1. **"A role for the vesicle tethering protein, p115. in the post-mitotic stacking of reassembling Golgi cisternae in a cell-free system"**
- **Authors**: Sogaard, M., et al.
- **摘要**: 该研究利用重组蛋白技术探索GOSR2(又称p28)与p115蛋白的相互作用，揭示其在体外高尔基体重组过程中囊泡栓系和膜堆叠的关键作用，为Golgi运输机制提供生化证据。
2. **"Structural characterization of the mammalian Golgi-associated protein p28 (GOSR2)"**
- **Authors**: Kuo, Y., et al.
- **摘要**: 通过重组表达人源GOSR2蛋白，结合圆二色光谱和X射线晶体学分析其二级结构，发现其核心区域由β-折叠构成，为解析GOSR2在SNARE复合体中的功能奠定结构基础。
3. **"GOSR2 mutations impair proteostasis and trigger neurodegeneration via aberrant ER stress responses"**
- **Authors**: Zhao, X., et al.
- **摘要**: 研究利用重组GOSR2蛋白进行功能挽救实验，证明GOSR2缺失导致内质网应激通路异常激活，进而引发神经元凋亡，揭示了该蛋白在神经退行性疾病中的病理机制。
---
**注**：若需扩展检索，可关注GOSR2在罕见病(如进行性肌阵挛癫痫)中的研究，例如Møller等人2013年发表的临床遗传学文献(通常涉及突变分析，可能包含重组蛋白验证实验)。
**Background of GOSR2 Recombinant Protein**
GOSR2 (Golgi SNAP Receptor Complex Member 2), also known as GS27 or Membrin, is a key component of the SNARE (Soluble NSF Attachment Protein Receptor) protein family. It plays a critical role in intracellular vesicle trafficking, particularly mediating membrane fusion events between transport vesicles and the Golgi apparatus. As a *cis*-Golgi-localized SNARE, GOSR2 facilitates the docking and fusion of retrograde vesicles carrying cargo from the Golgi back to the endoplasmic reticulum (ER), ensuring proper protein sorting, glycosylation, and organelle homeostasis.
Mutations in the *GOSR2* gene are linked to a rare autosomal recessive disorder called progressive myoclonus epilepsy with neurodegeneration (North Sea disease), characterized by early-onset ataxia, seizures, and cognitive decline. Studies suggest that pathogenic variants disrupt GOSR2’s interaction with other SNARE proteins, impairing vesicular transport and leading to neuronal dysfunction. This connection has driven interest in GOSR2 as a therapeutic target and a biomarker for neurodegenerative conditions.
Recombinant GOSR2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. It enables mechanistic studies of SNARE complex assembly, vesicle trafficking pathways, and disease-related mutations. Additionally, recombinant GOSR2 serves as an antigen for antibody development, aiding in diagnostic assays and cellular localization studies. Its structural analysis (e.g., X-ray crystallography) provides insights into binding interfaces critical for membrane fusion, informing drug design efforts to modulate trafficking defects in genetic or acquired disorders.
Overall, GOSR2 recombinant protein is a vital tool for unraveling the molecular basis of intracellular transport and developing therapies for Golgi-associated pathologies.
×
