Mouse Monoclonal NT5E Antibody

中文名： NT5E抗体
别名： NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

货号: IPD30614

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/500 - 1/2000	Human,Mouse,Rat,Rabbit
IF	咨询技术	Human,Mouse,Rat,Rabbit
IHC	1/200 - 1/1000	Human,Mouse,Rat,Rabbit
ICC	技术咨询	Human,Mouse,Rat,Rabbit
FCM	1/200 - 1/400	Human,Mouse,Rat,Rabbit
Elisa	1/10000	Human,Mouse,Rat,Rabbit

产品详情

Aliases	NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Entrez GeneID	4907
clone	4G6E3
WB Predicted band size	63.4kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Rabbit
Immunogen	Purified recombinant fragment of human NT5E (AA: 30-250) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using NT5E mouse mAb against A431 cell lysate.
Flow cytometric analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using NT5E mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using NT5E mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse testis using NT5E mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat kidney using NT5E mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit testis using NT5E mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit spleen using NT5E mouse mAb with DAB staining.

参考文献

以下是关于NT5E抗体的3篇文献示例(文献信息为虚构，仅供示例参考)：

1. **文献名称**：NT5E抗体通过抑制CD73活性增强抗肿瘤免疫反应

**作者**：Smith A, et al.

**摘要**：研究证明，靶向NT5E(CD73)的单克隆抗体可有效阻断其胞外酶活性，减少腺苷生成，从而逆转肿瘤微环境的免疫抑制，增强T细胞介导的抗肿瘤反应。

2. **文献名称**：CD73在结直肠癌中的表达及NT5E抗体的诊断价值

**作者**：Zhang L, et al.

**摘要**：该研究通过免疫组化分析发现NT5E在结直肠癌组织中高表达，并开发了一种高特异性NT5E抗体，证实其可作为潜在的诊断标志物和治疗靶点。

3. **文献名称**：靶向NT5E的抗体-药物偶联物在乳腺癌治疗中的应用

**作者**：Johnson R, et al.

**摘要**：研究构建了一种NT5E抗体-化疗药物偶联物，在乳腺癌模型中显著抑制肿瘤生长，且毒性低于传统化疗，提示其临床转化潜力。

(注：以上文献为示例，实际引用需以真实发表的论文为准。)

背景信息

NT5E, also known as CD73. is a cell surface ectoenzyme encoded by the NT5E gene. It catalyzes the conversion of extracellular adenosine monophosphate (AMP) to adenosine, a potent immunosuppressive molecule that modulates immune responses by binding to adenosine receptors on immune cells. NT5E plays a critical role in tumor microenvironment regulation, where its overexpression is linked to immune evasion, angiogenesis, and cancer progression. In many cancers, elevated NT5E levels correlate with poor prognosis and resistance to therapies, including immune checkpoint inhibitors.

NT5E antibodies are tools designed to target and inhibit the enzymatic activity or signaling of CD73. These antibodies are primarily used in research to study the biological functions of CD73 in cancer immunology, inflammation, and autoimmune diseases. Therapeutically, NT5E-blocking antibodies are under investigation as potential cancer immunotherapies, aiming to suppress adenosine-mediated immunosuppression and enhance anti-tumor T-cell activity. Preclinical studies show that combining NT5E antibodies with other immunotherapies (e.g., anti-PD-1/PD-L1) may synergistically improve tumor control. Several anti-CD73 antibodies, such as oleclumab and CPI-006. have entered clinical trials, demonstrating safety and preliminary efficacy in solid tumors. Despite promising results, challenges remain in optimizing target specificity, minimizing off-target effects, and understanding resistance mechanisms. Overall, NT5E antibodies represent a growing area of interest in both experimental and clinical oncology.