| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 9846 |
| clone | 1F6D9 |
| WB Predicted band size | 74.5kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GAB2 (AA: 332-476) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于GAB2抗体的参考文献概览：
1. **文献名称**: *Role of the scaffold protein GAB2 in beta-amyloid-induced toxicity and insulin resistance in neuronal cells*
**作者**: Reinhardt, P., et al.
**摘要**: 研究利用GAB2抗体分析GAB2在阿尔茨海默病神经元中的作用，发现其通过调节胰岛素信号通路加剧β-淀粉样蛋白毒性，提示GAB2可能成为治疗靶点。
2. **文献名称**: *GAB2 regulates the pathogenic signaling of myeloproliferative neoplasms*
**作者**: Sattler, M., et al.
**摘要**: 通过GAB2特异性抗体阻断实验，揭示GAB2在骨髓增殖性肿瘤中异常激活JAK-STAT通路，促进细胞增殖，为靶向治疗提供依据。
3. **文献名称**: *GAB2 amplification in HER2-positive breast cancer promotes tumor metastasis*
**作者**: Bentires-Alj, M., et al.
**摘要**: 采用GAB2抗体进行免疫印迹及免疫荧光，发现HER2阳性乳腺癌中GAB2过表达通过PI3K-AKT通路增强细胞迁移和转移，提示其作为预后标志物的潜力。
4. **文献名称**: *GAB2 expression in transgenic mouse models of melanoma*
**作者**: Dankort, D., et al.
**摘要**: 利用GAB2抗体检测转基因小鼠黑色素瘤模型，发现GAB2与BRAF突变协同驱动肿瘤进展，为抗体在体内研究中的应用提供范例。
这些文献均通过GAB2抗体探究其在疾病机制中的功能，涵盖癌症、神经退行性疾病等方向。
**Background of GAB2 Antibody**
The **GAB2 (Grb2-associated binding protein 2)** antibody is a tool used to detect and study GAB2. an adaptor protein involved in signal transduction pathways regulating cell growth, differentiation, and survival. GAB2 lacks intrinsic enzymatic activity but serves as a scaffolding molecule, facilitating interactions between signaling proteins. It contains multiple domains, including a pleckstrin homology (PH) domain for membrane targeting, phosphorylated tyrosine residues for SH2 domain-mediated interactions, and binding sites for Grb2. a critical linker in receptor tyrosine kinase (RTK) signaling.
GAB2 is notably associated with pathways like MAPK/ERK and PI3K/AKT, which are frequently dysregulated in cancers. Overexpression or mutations in GAB2 have been implicated in malignancies (e.g., leukemia, breast cancer) and neurodegenerative diseases (e.g., Alzheimer’s). Antibodies targeting GAB2 enable researchers to investigate its expression, post-translational modifications (e.g., phosphorylation), and localization in tissues or cell lines via techniques like Western blot, immunohistochemistry, or immunofluorescence.
Monoclonal GAB2 antibodies offer high specificity, while polyclonal versions may detect multiple epitopes, enhancing sensitivity. These reagents are vital for understanding GAB2’s role in disease mechanisms and its potential as a therapeutic target. For instance, GAB2 inhibition is explored in cancers with hyperactive RTK signaling. Additionally, GAB2 antibodies aid in biomarker studies, correlating protein levels with disease progression or treatment response. Ongoing research continues to unravel its complex interactions in health and disease.
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