| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BMK1; ERK4; ERK5; PRKM7 |
| Entrez GeneID | 5598 |
| clone | 8H1D5 |
| WB Predicted band size | 88.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAPK7 (AA: 411-556) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAPK7(ERK5)抗体的3篇参考文献示例(文献名称、作者及摘要概括)：
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1. **文献名称**：*"ERK5/MAPK7 promotes tumorigenesis in triple-negative breast cancer via vascular mimicry"*
**作者**：Sawano et al. (2018)
**摘要**：研究利用MAPK7特异性抗体进行Western blot和免疫组化，发现ERK5在乳腺癌中通过调控血管生成拟态促进肿瘤侵袭，提示其作为潜在治疗靶点。
2. **文献名称**：*"ERK5 regulates endothelial cell apoptosis in atherosclerosis via NF-κB signaling"*
**作者**：Zheng et al. (2019)
**摘要**：通过MAPK7抗体敲低实验，证明ERK5通过抑制NF-κB通路保护内皮细胞凋亡，为动脉粥样硬化机制提供新见解。
3. **文献名称**：*"MAPK7 antibody-based detection reveals its role in inflammatory bowel disease pathogenesis"*
**作者**：Chen et al. (2020)
**摘要**：利用抗MAPK7抗体进行组织染色和流式分析，揭示ERK5在肠道炎症中通过调控巨噬细胞极化加剧疾病进展。
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*注：以上为示例文献，实际引用需核对真实论文信息。建议通过PubMed或Google Scholar搜索关键词“MAPK7 antibody”“ERK5”获取具体文献。*
The mitogen-activated protein kinase 7 (MAPK7), also known as extracellular signal-regulated kinase 5 (ERK5), is a member of the MAPK family involved in critical cellular processes such as proliferation, differentiation, and survival. Unlike other MAPKs, MAPK7 contains a unique C-terminal tail that regulates its activation and nuclear translocation. It is primarily activated by dual phosphorylation of threonine and tyrosine residues in the T-E-Y motif by upstream kinases like MEK5. MAPK7 plays a key role in stress responses, angiogenesis, and developmental pathways, with dysregulation linked to cancer, cardiovascular diseases, and neurodegenerative disorders.
MAPK7 antibodies are essential tools for detecting and studying the expression, localization, and activation status of MAPK7 in various biological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate MAPK7 signaling dynamics. Phospho-specific MAPK7 antibodies targeting the phosphorylated T-E-Y motif are particularly valuable for assessing its activation state. Research applications include exploring MAPK7's role in tumor progression, where its overexpression in certain cancers correlates with poor prognosis, or in cardiovascular studies examining its impact on endothelial cell function. Validating antibody specificity using knockout/knockdown controls is critical to ensure reliable experimental outcomes, as cross-reactivity with other MAPKs can occur. Overall, MAPK7 antibodies are pivotal for unraveling the protein's diverse physiological and pathological functions.
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