| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 2932 |
| clone | 3D6B4 |
| WB Predicted band size | 46.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GSK3B抗体的参考文献示例(内容为虚构，仅作格式参考)：
1. **"GSK3β Antibody Validation in Neurodegenerative Disease Models"**
*作者：Lee et al. (2018)*
摘要：本研究验证了多种GSK3B抗体的特异性，通过Western blot和免疫组化分析阿尔茨海默病小鼠模型中GSK3B的磷酸化(Ser9)及总蛋白表达变化，证实抗体在区分活性与非活性形式中的可靠性。
2. **"Role of GSK3β in Wnt Signaling: Antibody-Based Detection in Colorectal Cancer"**
*作者：Zhang et al. (2015)*
摘要：利用特异性GSK3B抗体探究结直肠癌组织中GSK3B的亚细胞定位及活性状态，发现核内GSK3B水平升高与β-catenin异常激活相关，为靶向治疗提供依据。
3. **"Comparative Study of Commercial GSK3β Antibodies for Phospho-Specific Epitopes"**
*作者：Martinez & Kumar (2020)*
摘要：系统评估了6种商品化抗体的性能，发现部分抗体存在交叉反应性，推荐使用克隆号7B10用于人源组织p-GSK3β(Tyr216)的特异性检测。
4. **"GSK3β Inhibition in Diabetes: Antibody-Driven Quantification of Hepatic Isoform Activity"**
*作者：Chen et al. (2016)*
摘要：通过免疫沉淀结合活性检测，揭示2型糖尿病模型中肝脏GSK3B活性显著上调，抗体特异性抑制实验证实其调控糖原合成的关键作用。
(注：以上文献为示例性质，实际引用请查询真实数据库。)
Glycogen synthase kinase 3 beta (GSK3B) is a serine/threonine kinase involved in multiple signaling pathways, including Wnt, insulin, and growth factor signaling. It plays critical roles in cellular processes such as glycogen metabolism, cell proliferation, differentiation, and apoptosis. Dysregulation of GSK3B has been linked to neurodegenerative diseases (e.g., Alzheimer’s disease), cancer, diabetes, and mood disorders. Antibodies targeting GSK3B are essential tools for studying its expression, activity, and post-translational modifications in these contexts.
GSK3B exists in two isoforms (α and β) and is regulated by phosphorylation at specific residues. For example, phosphorylation at Ser9 inhibits its activity, while Tyr216 phosphorylation enhances it. Antibodies detecting total GSK3B or its phosphorylated forms (e.g., anti-pSer9-GSK3B or anti-pTyr216-GSK3B) are widely used to assess its functional state in experimental models. These antibodies are applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore GSK3B's role in disease mechanisms or drug responses.
Due to its involvement in tau phosphorylation (a hallmark of Alzheimer’s) and β-catenin degradation (linked to cancer), GSK3B antibodies are particularly valuable in neuroscience and oncology research. Validation of antibody specificity via knockout controls or peptide competition is crucial, as cross-reactivity with related kinases (e.g., GSK3A) may occur. Commercial GSK3B antibodies often specify reactivity across species (human, mouse, rat) and provide application-specific optimization data.
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