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Mouse Monoclonal HH3 Antibody

  • 中文名: HH3抗体
  • 别    名: HIST2H3A;H3/n; H3/o
货号: IPD30741
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500 - 1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/200 - 1/400 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesHIST2H3A;H3/n; H3/o
Entrez GeneID333932
clone6D3B9
WB Predicted band size15.4kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenSynthesized peptide fragment of human HH3 (AA: 121-136) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是关于HH3(Histone H3)抗体的3篇参考文献,涵盖其在细胞周期及疾病研究中的应用:

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1. **文献名称**: *Phosphorylation of Histone H3 at Serine 10 Correlates with Chromosome Condensation During Mitosis*

**作者**: Wei, Y., Yu, L., Bowen, J., et al.

**摘要**: 该研究通过免疫荧光技术结合HH3抗体(针对H3S10磷酸化修饰),揭示了组蛋白H3第10位丝氨酸磷酸化(H3S10ph)在有丝分裂早期染色体凝聚中的关键作用,证实其可作为细胞周期进展的分子标记。

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2. **文献名称**: *Mitosis-specific Phosphorylation of Histone H3 Initiates Centromere Assembly*

**作者**: Hendzel, M.J., Wei, Y., Mancini, M.A., et al.

**摘要**: 研究利用HH3抗体(特异性识别H3T3磷酸化修饰)发现,组蛋白H3的苏氨酸3位点(H3T3ph)磷酸化是启动着丝粒组装的关键信号,并证实该修饰对有丝分裂中染色体的正确分离至关重要。

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3. **文献名称**: *H3K27me3 Antibodies as a Tool to Study Epigenetic Regulation in Cancer*

**作者**: Cao, R., Bannister, A.J., & Kouzarides, T.

**摘要**: 该文献探讨了针对H3K27三甲基化(H3K27me3)的HH3抗体在癌症表观遗传调控研究中的应用,发现该修饰通过Polycomb蛋白复合物抑制肿瘤抑制基因表达,与多种癌症的恶性进展相关。

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4. **文献名称**: *HH3 as a Proliferation Marker in Glioblastoma*

**作者**: Ribalta, T., McCutcheon, I.E., & Aldape, K.D.

**摘要**: 研究通过免疫组化技术使用HH3抗体(识别磷酸化H3S28)评估胶质母细胞瘤的细胞增殖活性,发现其表达水平与肿瘤分级及患者预后显著相关,提示其作为临床病理学标志物的潜力。

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以上文献展示了HH3抗体在细胞周期机制、表观遗传学及癌症研究中的广泛应用。

背景信息

The HH3 antibody specifically targets histone H3. a core component of nucleosomes that play a critical role in chromatin structure and epigenetic regulation. Histone H3 undergoes various post-translational modifications (e.g., phosphorylation, acetylation, methylation) that influence chromatin dynamics, gene expression, and cellular processes like mitosis and DNA repair. The HH3 antibody is often used to detect phosphorylation at specific residues (e.g., Ser10. Ser28), which are biomarkers of mitotic cells, making it valuable in studying cell proliferation, cancer biology, and developmental processes. In research, it is widely applied in techniques such as immunofluorescence, Western blotting, and flow cytometry to visualize mitotic progression or chromatin changes in diseases like cancer and neurodegenerative disorders. Additionally, HH3 antibodies help explore epigenetic mechanisms in stem cell differentiation, cellular senescence, and response to DNA damage. Their specificity for modified histone H3 variants also aids in understanding how chromatin remodeling contributes to disease pathogenesis or therapeutic resistance. Overall, HH3 antibodies serve as essential tools in both basic and translational research, bridging histone modification studies with clinical applications.

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