| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 2549 |
| clone | 5F11C8 |
| WB Predicted band size | 32.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GAB1 (AA: 661-724) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与GAB1抗体相关的代表性文献摘要(基于公开研究整理，非实时数据库检索)：
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1. **文献名称**: *"Essential role of Gab1 for signaling by the Met receptor in vivo"*
**作者**: Suzuki et al.
**摘要**: 本研究利用基因敲除小鼠模型，发现GAB1在Met受体介导的胚胎发育和肝细胞生长中起关键作用。通过特异性抗体检测GAB1蛋白表达，证实其与Met受体信号通路的相互作用，并揭示GAB1缺失导致细胞迁移和器官形成缺陷。
2. **文献名称**: *"The Gab1 PH domain is required for localization of Gab1 at sites of cell-cell contact and epithelial morphogenesis downstream of the met receptor tyrosine kinase"*
**作者**: Maroun et al.
**摘要**: 研究通过免疫荧光技术(使用GAB1抗体)分析GAB1蛋白在细胞间的定位，发现其PH结构域对上皮细胞形态发生和Met受体介导的细胞间粘附至关重要，为癌症转移机制提供了分子依据。
3. **文献名称**: *"A Grb2-associated docking protein in EGF- and insulin-receptor signalling"*
**作者**: Holgado-Madruga et al.
**摘要**: 首次鉴定GAB1作为EGFR和胰岛素受体信号通路中的关键适配蛋白，通过Western blot和免疫沉淀实验(使用GAB1抗体)证明其通过结合Grb2调控下游MAPK和PI3K通路，影响细胞增殖和存活。
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**注**：以上文献均涉及GAB1抗体的实验应用(如Western blot、免疫沉淀等)，聚焦于其在生长因子信号转导、癌症和发育生物学中的功能。如需具体抗体品牌或实验细节，需结合文献补充材料或抗体公司说明书。
GAB1 (GRB2-associated binding protein 1) is a key adaptor/scaffolding protein involved in signal transduction downstream of receptor tyrosine kinases (RTKs) and cytokine receptors. It plays a critical role in mediating cellular responses to growth factors (e.g., EGF, HGF) and extracellular signals by recruiting downstream effectors like PI3K, SHP2. and CRKL through multiple phosphorylation sites and protein interaction domains. GAB1’s interaction with GRB2 facilitates its localization to activated receptors, enabling the activation of pathways such as PI3K/AKT and MAPK, which regulate cell proliferation, differentiation, and survival. Dysregulation of GAB1 is linked to cancer, developmental disorders, and immune dysfunction.
GAB1 antibodies are essential tools for studying its expression, phosphorylation status, and interactions in diseases. Researchers use them in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate GAB1’s role in signaling networks or its aberrant activity in tumors (e.g., breast, lung cancers). Specific antibodies targeting phosphorylated residues (e.g., Tyr627. Tyr659) help analyze pathway activation. Commercial GAB1 antibodies are typically validated in knock-out cell lines to confirm specificity. However, cross-reactivity with homologous proteins (e.g., GAB2) requires careful experimental controls.
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