SARS-CoV-2 Neutralizing Antibody ELISA Kit

货号: IPD-EL33243HU

Price： ￥2500

48T 36T

数量：

大包装询价

产品描述：
新型冠状病毒(SARS-CoV-2)中和抗体酶联免疫试剂盒为竞争法ELISA试剂盒，定性检测血清、血浆样本中的SARS-CoV-2 neutralizing Ab含量。SARS-CoV-2 neutralizing Ab即新冠病毒中和抗体。背景上，新冠疫情全球大流行促使其研究。机制方面，中和抗体可识别并结合新冠病毒刺突蛋白，阻止病毒与宿主细胞受体结合，阻断感染过程，是抗病毒研究关键方向。为疫苗研发、免疫应答评估及感染康复者抗体动态研究提供重要依据；适用于科研场景中疫苗接种后抗体效价追踪、群体免疫水平研究或病毒感染动物模型的免疫效果评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2
缩写：
SARS-CoV-2 neutralizing Ab
种属：
Homo sapiens (Human)
样本类型：
serum, plasma
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Infectious Diseases
测定原理：
qualitative
测定方法：
Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

One 96-well Microtiter plate ( coated Microwells) -- This microwell plate has been pre-coated with recombinant SARS-CoV-2 S RBD antigen and can be dismountable into 12 strips of 8 wells.
A bottle of Standard (10 x concentrate; 200 μl/vial) -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
A bottle of HRP-conjugated ACE2 (100 x concentrate; 60 μl/vial) -- ACE2 interacts with SARS-CoV-2 S RBD, mimicking the process of SARS-CoV-2 entry into the host cells. HRP catalyzes the TMB Substrate solution, eliciting a chromogenic reaction.
A bottle of HRP-conjugate Diluent (10 ml/vial) -- Dilute the HRP-conjugated ACE2 solution.
Two bottles of Sample Diluent (20 ml/vial) -- Reconstitute the standard and dilute the sample to an appropriate concentration.
A bottle of Wash Buffer (25 x concentrate; 20 ml/vial) -- Wash away unbound or free substances.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer.
Absorbent paper for blotting the microtiter plate.

大包装询价