| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45 |
| Entrez GeneID | 2778 |
| clone | 7G6G5 |
| WB Predicted band size | 45.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GNAS (AA: 42-188) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GNAS抗体的参考文献示例(内容为虚构示例，仅用于演示格式)：
1. **《GNAS蛋白在假性甲状旁腺功能减退症中的表达分析》**
作者：Smith A, et al.
摘要：本研究通过免疫组化技术，使用特异性GNAS抗体检测假性甲状旁腺功能减退症患者组织样本，发现GNAS蛋白表达显著降低，提示其与疾病中G蛋白信号通路异常相关。
2. **《GNAS抗体在肿瘤突变检测中的比较研究》**
作者：Lee C, et al.
摘要：文章对比了三种商用GNAS抗体(克隆号XX、YY、ZZ)的灵敏度和特异性，发现克隆号XX抗体在识别GNAS R201H突变蛋白中表现最优，适用于临床样本的免疫印迹分析。
3. **《GNAS基因突变与抗体检测技术在胃肠肿瘤中的应用》**
作者：Garcia R, et al.
摘要：研究通过免疫组织化学和测序技术，验证了GNAS抗体在结直肠癌中检测激活突变的有效性，证实GNAS突变与肿瘤侵袭性相关，并推荐抗体作为辅助诊断工具。
4. **《GNAS信号通路中抗体干扰对细胞功能的影响》**
作者：Wang T, et al.
摘要：利用GNAS中和抗体阻断Gαs蛋白活性，发现其可抑制cAMP信号传导并减缓特定癌细胞的增殖，为靶向治疗提供实验依据。
(注：以上文献为模拟内容，实际引用需查询真实数据库如PubMed。)
The GNAS antibody targets proteins encoded by the GNAS complex locus, a gene critical in G protein-coupled receptor (GPCR) signaling. GNAS produces multiple transcripts through alternative splicing and imprinting, with the most studied being the stimulatory G-protein α-subunit (Gsα). Gsα activates adenylate cyclase, generating cAMP, a key second messenger regulating cellular processes like proliferation and hormone response.
GNAS mutations or dysregulation are linked to diseases. Activating mutations (e.g., R201H) cause McCune-Albright syndrome (fibrous dysplasia, endocrine disorders) and sporadic endocrine tumors. In contrast, maternally inherited inactivating mutations lead to pseudohypoparathyroidism type 1A (PHP1A), characterized by hormone resistance, while paternal mutations result in pseudopseudohypoparathyroidism (PPHP) without endocrine abnormalities.
Antibodies against GNAS are vital tools in research and diagnostics. They help detect Gsα expression or post-translational modifications in tissues, aiding studies on GPCR signaling defects. In clinical settings, they assist in diagnosing GNAS-related disorders or identifying somatic mutations in tumors. However, challenges remain due to the locus’s complexity, including multiple isoforms and imprinting-specific expression, requiring careful validation of antibody specificity. Overall, GNAS antibodies contribute to understanding disease mechanisms and developing targeted therapies.
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