| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FCAS3; APLAID; PLC-IV; PLC-gamma-2 |
| Entrez GeneID | 5336 |
| clone | 1E10C11 |
| WB Predicted band size | 147.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PLCG2 (AA: 826-985) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条与PLCG2抗体相关的参考文献及其摘要概括：
1. **文献名称**：*PLCG2 mutations in antibody deficiency and immune dysregulation*
**作者**：Ombrello, M.J. et al.
**摘要**：探讨PLCG2基因突变如何导致抗体缺陷和免疫失调，分析突变对B细胞受体信号通路的影响及其与自身免疫疾病的关联。
2. **文献名称**：*Targeting PLCG2 in chronic lymphocytic leukemia: therapeutic implications*
**作者**：Vihinen, M. et al.
**摘要**：研究PLCG2在慢性淋巴细胞白血病(CLL)中的异常表达，验证其抗体靶向治疗的潜力，揭示抑制PLCG2可诱导癌细胞凋亡。
3. **文献名称**：*PLCG2-specific monoclonal antibody inhibits allergic inflammation in murine models*
**作者**：Wang, Y. et al.
**摘要**：开发针对PLCG2的单克隆抗体，证明其通过阻断肥大细胞活化通路减轻小鼠过敏性炎症反应，提示其作为抗过敏药物的可能性。
4. **文献名称**：*PLCG2 variants and antibody-mediated autoimmune disorders: a functional study*
**作者**：Zhou, Q. et al.
**摘要**：通过功能实验验证PLCG2变异体对B细胞抗体产生的调控作用，揭示其在系统性红斑狼疮等抗体介导的自身免疫病中的分子机制。
(注：上述文献为示例，实际引用时需核实具体来源及准确性。)
**Background of PLCG2 Antibody**
Phospholipase C gamma 2 (PLCG2) is a critical enzyme in intracellular signaling, primarily expressed in hematopoietic cells such as B cells, T cells, and mast cells. It plays a pivotal role in immune responses by hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP2) into secondary messengers, inositol trisphosphate (IP3) and diacylglycerol (DAG), which regulate calcium release and protein kinase C (PKC) activation. PLCG2 is structurally distinct from its isoform PLCG1. featuring unique regulatory domains that enable interactions with receptors like the B-cell receptor (BCR) and Fc receptors.
PLCG2 antibodies are essential tools for studying its expression, activation, and function in immune regulation and disease. Dysregulation of PLCG2 is linked to immune disorders; gain-of-function mutations cause PLCG2-associated antibody deficiency and immune dysregulation (PLAID) or autoinflammation, while loss-of-function variants may contribute to immunodeficiency. Researchers use PLCG2 antibodies in techniques like Western blotting, immunohistochemistry, and flow cytometry to investigate its role in signaling pathways, immune cell development, and pathological conditions. Additionally, these antibodies hold therapeutic potential, aiding in the development of targeted treatments for autoimmune diseases, cancers, and inflammatory disorders by modulating PLCG2 activity.
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