| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KOX; KOX-1; RENOX |
| Entrez GeneID | 50507 |
| clone | 3H2C4 |
| WB Predicted band size | 67kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NOX4 (AA: 210-310) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NOX4抗体的3篇参考文献，按作者和内容概括整理：
1. **文献名称**：*"NOX4 NADPH oxidase mediates fibrosis and inflammation in diabetic nephropathy"*
**作者**：Gorin Y, Block K
**摘要**：该研究探讨了NOX4在糖尿病肾病中的作用，通过Western blot和免疫组化验证了NOX4抗体的特异性。结果显示，NOX4在肾纤维化中高表达，其抗体信号在NOX4敲除小鼠中显著减弱，证实了抗体的可靠性。
2. **文献名称**：*"Validation of a specific commercial antibody for NADPH oxidase 4 (NOX4) localization in human tissues"*
**作者**：Sirokmány G, Geiszt M
**摘要**：研究系统评估了多种商业NOX4抗体的特异性，发现部分抗体存在交叉反应问题。通过CRISPR-Cas9敲除细胞系验证了某品牌抗体的特异性，并成功应用于人血管内皮组织中的NOX4定位分析。
3. **文献名称**：*"NOX4 promotes ferroptosis in astrocytes via lipid peroxidation during ischemic stroke"*
**作者**：Chen L, Li X
**摘要**：利用NOX4抗体(Abcam货号ab154244)检测缺血性脑卒中模型中星形胶质细胞的NOX4表达，结合流式细胞术和铁死亡抑制剂实验，证实NOX4通过脂质过氧化调控铁死亡过程。抗体特异性通过siRNA干扰实验验证。
这些文献涵盖了NOX4抗体的疾病机制研究、特异性验证及实验应用场景。如需扩展，可进一步检索近年关于NOX4亚细胞定位或翻译后修饰检测的抗体优化研究。
The NADPH oxidase 4 (NOX4) antibody is a crucial tool for studying the NOX4 enzyme, a member of the NADPH oxidase family that generates reactive oxygen species (ROS), primarily hydrogen peroxide (H2O2). Unlike other NOX isoforms, NOX4 is constitutively active and does not require cytosolic regulatory subunits for activation. It is expressed in various tissues, including kidneys, blood vessels, and the pancreas, playing roles in cellular signaling, differentiation, and stress responses. Dysregulation of NOX4 has been implicated in pathological processes such as fibrosis, cancer, inflammation, and cardiovascular diseases.
NOX4 antibodies are widely used to detect NOX4 protein expression, localization, and regulation in both physiological and disease contexts. They enable researchers to investigate NOX4's involvement in oxidative stress-mediated damage, cellular senescence, and tissue remodeling through techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity and validation of NOX4 antibodies are critical, as cross-reactivity with other NOX isoforms or non-specific binding can lead to inaccurate interpretations.
Research utilizing NOX4 antibodies has advanced understanding of its dual roles—acting as a mediator of redox signaling in homeostasis while contributing to oxidative injury in diseases like diabetic nephropathy, pulmonary fibrosis, and atherosclerosis. These studies highlight NOX4 as a potential therapeutic target, driving the development of selective inhibitors. Reliable NOX4 antibodies remain essential for unraveling its complex biology and translational applications.
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