| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | SIR2L4 |
| Entrez GeneID | 23409 |
| clone | 6G6D2 |
| WB Predicted band size | 35.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SIRT4 (AA: 215-314) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与SIRT4抗体相关的研究文献及其摘要概括：
1. **"SIRT4 Inhibits Glutamate Dehydrogenase and Opposes the Effects of Calorie Restriction in Pancreatic Beta Cells"**
- **作者**: Haigis, M.C. 等 (2006)
- **摘要**: 该研究揭示了SIRT4通过ADP-核糖基化抑制谷氨酸脱氢酶(GDH)活性，调节胰岛素分泌。文中使用特异性SIRT4抗体进行Western blot和免疫沉淀实验，验证了其在胰岛β细胞中的线粒体定位及蛋白表达水平。
2. **"SIRT4 Has Tumor-Suppressive Activity and Regulates the Cellular Metabolic Response to DNA Damage by Inhibiting Mitochondrial Glutamine Metabolism"**
- **作者**: Jeong, S.M. 等 (2013)
- **摘要**: 研究发现SIRT4通过抑制谷氨酰胺代谢发挥肿瘤抑制功能，并参与DNA损伤应答。研究采用SIRT4抗体进行免疫印迹和免疫荧光实验，证实SIRT4在多种癌细胞系中的表达缺失及其与代谢重编程的关系。
3. **"Epigenetic Silencing of SIRT4 in Colorectal Cancer"**
- **作者**: Ahuja, N. 等 (2014)
- **摘要**: 该研究报道结直肠癌中SIRT4因启动子甲基化而表达沉默，且与患者预后不良相关。通过SIRT4特异性抗体进行免疫组化分析，显示肿瘤组织中SIRT4蛋白表达显著降低，支持其作为潜在生物标志物的可能性。
这些文献均利用SIRT4抗体在分子机制或疾病模型中探究其功能，涵盖代谢调控、肿瘤抑制及表观遗传调控等领域。
SIRT4 (Sirtuin 4) is a member of the sirtuin family of NAD+-dependent deacylases, primarily localized in mitochondria. It plays a critical role in regulating metabolic pathways, including fatty acid oxidation, amino acid metabolism, and insulin secretion. Unlike other sirtuins (e.g., SIRT1 or SIRT3), SIRT4 exhibits weak deacetylase activity but primarily functions as an ADP-ribosyltransferase, modulating enzyme activities to maintain metabolic homeostasis. Its involvement in suppressing tumor growth, promoting DNA repair, and influencing cellular stress responses has linked SIRT4 to aging, cancer, and metabolic disorders like diabetes.
SIRT4 antibodies are essential tools for detecting and quantifying SIRT4 protein levels in research. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA to study SIRT4 expression patterns across tissues, its subcellular localization, and interactions with metabolic enzymes. High-quality SIRT4 antibodies are validated for specificity, often using knockout (KO) models to confirm minimal cross-reactivity with other sirtuins. These antibodies enable investigations into SIRT4's role in mitochondrial dysfunction, cancer progression (e.g., as a tumor suppressor in certain cancers), and metabolic syndrome. Commercial SIRT4 antibodies are typically raised against conserved epitopes, available as monoclonal or polyclonal variants, and require optimization for experimental conditions. Recent studies leveraging these reagents highlight SIRT4’s therapeutic potential, driving demand for reliable antibodies in both basic and translational research.
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