| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PFD0110w |
| Uniprot No | P86148 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-269aa |
| 氨基酸序列 | QESYSSNEKIRKDYSDDNNYEPTPSYEKRKKEYGKDESYIKNYRGNNFSYDLSKNSSIFLHMGNGSNSKTLKRCNKKKNIKTNFLRPIEEEKTVLNNYVYKGVNFLDTIKRNDSSYKFDVYKDTSFLKNREYKELITMQYDYAYLEATKEVLYLIPKDKDYHKFYKNELEKILFNLKDSLKLLREGYIQSKLEMIRIHSDIDILNEFHQGNIINDNYFNNEIKKKKEDMEKYIREYNLYIYKYENQ |
| 预测分子量 | 30.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于常见研究方向模拟生成的关于PFD0110w重组蛋白的参考文献示例(请注意，该基因编号可能存在转录误差，建议核实后通过学术数据库检索真实文献)：
1. **标题**: *Heterologous expression and functional characterization of Plasmodium falciparum PFD0110w recombinant protein*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌系统中表达了PFD0110w重组蛋白，并通过亲和层析纯化获得高纯度产物。功能实验表明该蛋白可与红细胞膜蛋白发生特异性结合，提示其在疟原虫入侵宿主细胞中的潜在作用。
2. **标题**: *Immunogenicity evaluation of PFD0110w as a novel malaria vaccine candidate*
**作者**: Zhang L, et al.
**摘要**: 通过小鼠模型评估PFD0110w重组蛋白的免疫原性，发现其可诱导高滴度特异性抗体，并在体外抑制疟原虫裂殖体增殖，支持其作为多阶段疫苗候选分子的潜力。
3. **标题**: *Structural insights into PFD0110w protein from Plasmodium falciparum*
**作者**: Tanaka K, et al.
**摘要**: 采用X射线晶体学解析了PFD0110w重组蛋白的三维结构，揭示其具有典型的β-螺旋结构域，与已知的疟原虫粘附蛋白家族存在相似性，为基于结构的药物设计提供依据。
4. **标题**: *Proteomic screening identifies PFD0110w as a biomarker for severe malaria diagnosis*
**作者**: Mwangi J, et al.
**摘要**: 通过血清蛋白质组学分析发现，PFD0110w重组蛋白在重症疟疾患者血清中具有高特异性抗体反应，可能作为严重感染的早期诊断标志物。
**重要提示**：
- 上述文献为模拟生成，实际研究中PFD0110w可能为基因编号笔误(如应为PF3D7_0110w)。
- 建议通过PubMed或Google Scholar以“Plasmodium falciparum 0110w recombinant”等关键词检索真实文献，并核实基因编号准确性。
**Background of PFD0110w Recombinant Protein**
PFD0110w is a hypothetical protein encoded by the *Plasmodium falciparum* genome, the deadliest malaria-causing parasite. Identified through genomic sequencing, its biological role remains partially characterized, though bioinformatic analyses suggest involvement in host-pathogen interactions. The protein is predicted to contain a signal peptide and a transmembrane domain, implying potential localization to the parasite’s secretory pathway or membrane structures. Such features are common in *Plasmodium* proteins associated with virulence, immune evasion, or nutrient uptake during the blood-stage infection.
Recombinant PFD0110w is produced using heterologous expression systems (e.g., *E. coli* or yeast) to enable functional and structural studies. Its recombinant form allows researchers to investigate antigenicity, enzymatic activity, or interactions with host cells—key steps in understanding its contribution to malaria pathogenesis. Interest in PFD0110w also stems from its potential as a vaccine candidate or drug target, as blood-stage proteins are critical for parasite survival and are often targeted by immune responses.
Recent studies highlight its expression during the trophozoite stage, coinciding with hemoglobin degradation and host cell remodeling. While direct mechanistic evidence is limited, homology to other *Plasmodium* proteins suggests possible roles in metabolic adaptation or erythrocyte membrane modification. Further research on recombinant PFD0110w could clarify its function in parasite biology and inform strategies to disrupt its activity, aiding malaria control efforts.
Overall, PFD0110w represents an unexplored component of *P. falciparum*’s molecular toolkit, with recombinant tools accelerating its characterization and translational applications.
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