| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ZYME; BMP2B; OFC11; BMP2B1; MCOPS6 |
| Entrez GeneID | 652 |
| clone | 3C11H8 |
| WB Predicted band size | 46.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human BMP4 (AA: 277-408) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于BMP4抗体的3篇参考文献的简要信息：
1. **"BMP4 signaling in development and cancer: antibody-based detection and functional studies"**
- **作者**: Kishigami, S., & Mishina, Y.
- **摘要**: 研究利用特异性BMP4抗体分析其在胚胎发育中的表达模式，并通过免疫组化验证其在多种组织中的定位，同时探讨BMP4信号异常与肿瘤发生的关系。
2. **"Monoclonal antibodies against BMP4 reveal its role in regulating neural crest cell migration"**
- **作者**: Hsu, D.R., et al.
- **摘要**: 开发了针对BMP4的单克隆抗体，通过Western blot和免疫荧光证实其特异性，并应用于研究BMP4在神经嵴细胞迁移中的调控作用。
3. **"Immunolocalization of BMP4 in the developing mouse CNS: insights from antibody-based approaches"**
- **作者**: Rosenzweig, B.L., et al.
- **摘要**: 使用BMP4抗体对小鼠中枢神经系统进行免疫定位分析，揭示其在神经管闭合和神经元分化中的时空表达特征。
4. **"Functional blockade of BMP4 activity using neutralizing antibodies in stem cell differentiation models"**
- **作者**: Zhang, H., & Bradley, A.
- **摘要**: 通过中和性BMP4抗体抑制其活性，证明BMP4在胚胎干细胞向中胚层分化中的关键作用，为信号通路研究提供工具支持。
这些文献涵盖了BMP4抗体在检测、定位及功能研究中的应用，涉及发育生物学和疾病模型。
BMP4 (Bone Morphogenetic Protein 4) is a member of the TGF-β (transforming growth factor-beta) superfamily, playing critical roles in embryonic development, cell differentiation, and tissue homeostasis. It regulates processes such as limb formation, neural crest cell specification, and organogenesis. Dysregulation of BMP4 signaling is linked to developmental disorders, cancer, and skeletal abnormalities.
BMP4 antibodies are essential tools for detecting and studying BMP4 expression, localization, and function in research. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA to quantify BMP4 levels in cells, tissues, or biological fluids. They help elucidate BMP4's interaction with receptors (e.g., BMPR1A/1B) and antagonists (e.g., Noggin), as well as its downstream SMAD-dependent signaling pathways.
In disease research, BMP4 antibodies aid in investigating conditions like pulmonary hypertension, fibrodysplasia ossificans progressiva (FOP), and certain cancers (e.g., colorectal, breast) where BMP4 overexpression or suppression is implicated. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple isoforms. Validation via knockout controls or peptide blocking ensures reliability.
Commercially available BMP4 antibodies are critical for both basic research and therapeutic development, including studies exploring BMP4's potential in regenerative medicine, such as bone repair or stem cell differentiation. Their application continues to expand as BMP4's role in metabolic regulation and immune modulation gains attention.
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