| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COG5 |
| Uniprot No | Q9UP83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-257aa |
| 氨基酸序列 | MGWVGGRRRDSASPPGRSRSAADDINPAPANMEGGGGSVAVAGLGARGSGAAAATVRELLQDGCYSDFLNEDFDVKTYTSQSIHQAVIAEQLAKLAQGISQLDRELHLQVVARHEDLLAQATGIESLEGVLQMMQTRIGALQGAVDRIKAKIVEPYNKIVARTAQLARLQVACDLLRRIIRILNLSKRLQGQLQGGSREITKAAQSLNELDYLSQGIDLSGIEVIENDLLFIARARLEVENQAKRLLEQGLETQNPT |
| 预测分子量 | 33.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COG5重组蛋白的模拟参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库检索获取)：
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1. **文献名称**: "Functional Characterization of COG5 in Golgi Trafficking Using Recombinant Protein Expression"
**作者**: Smith A, et al.
**摘要**: 本研究通过重组表达COG5蛋白，揭示了其在维持高尔基体结构完整性中的关键作用。实验表明，COG5与COG复合体其他亚基相互作用，调控囊泡运输和糖蛋白修饰。
2. **文献名称**: "COG5 Deficiency Disrupts ER-Golgi Recycling: Insights from Recombinant COG5 Reconstitution"
**作者**: Lee J, Ungar D.
**摘要**: 通过重组COG5蛋白的体外功能分析，发现其缺失导致内质网-高尔基体逆向运输异常，并影响SNARE蛋白复合体的组装，为先天性糖基化疾病的机制提供新证据。
3. **文献名称**: "Structural Analysis of Recombinant COG5 Subunit and Its Role in the COG Complex"
**作者**: Zhang Y, et al.
**摘要**: 利用重组COG5蛋白的晶体结构解析，阐明了其与COG3、COG7亚基的互作界面，揭示了COG复合体在膜运输中的分子调控机制。
4. **文献名称**: "A Yeast Two-Hybrid Screen Identifies COG5 as a Novel Partner of Syntaxin 5 in Vesicle Docking"
**作者**: Lupashin V, et al.
**摘要**: 通过重组COG5蛋白的互作实验，发现其与Syntaxin 5直接结合，调控高尔基体膜融合过程，进一步验证COG复合体在囊泡锚定中的功能。
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建议通过PubMed、Google Scholar或Web of Science等平台，以关键词“COG5 recombinant protein”或“COG complex trafficking”检索最新文献，重点关注细胞生物学及糖基化疾病相关研究。
**Background of COG5 Recombinant Protein**
The Conserved Oligomeric Golgi (COG) complex is a critical component of intracellular trafficking, primarily regulating vesicle tethering and glycosylation processes within the Golgi apparatus. Comprising eight subunits (COG1–COG8), it is organized into two lobes (lobe A: COG1–4; lobe B: COG5–8). COG5. a subunit of lobe B, plays a pivotal role in maintaining Golgi structure, cargo sorting, and retrograde transport of glycosylation enzymes. Mutations in COG5 are linked to congenital disorders of glycosylation (CDGs), characterized by impaired protein and lipid glycosylation, leading to multisystemic symptoms.
COG5 recombinant protein is engineered to study the molecular mechanisms of COG complex dysfunction and its pathological implications. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*), it typically includes affinity tags (e.g., His-tag) for purification. This recombinant tool enables *in vitro* analyses, such as binding assays, structural studies, and functional rescue experiments in COG-deficient cell models.
Research applications include investigating COG5’s interactions with other trafficking machinery (e.g., SNAREs, Rab GTPases), dissecting its role in glycosylation pathways, and developing therapeutic strategies for CDGs. Additionally, it serves as an antigen for antibody production in diagnostic assays. By elucidating COG5’s role in Golgi dynamics, studies using recombinant COG5 contribute to broader insights into cellular homeostasis and disease mechanisms linked to membrane trafficking defects.
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