| WB | 咨询技术 | Human,Mouse,Monkey |
| IF | 咨询技术 | Human,Mouse,Monkey |
| IHC | 咨询技术 | Human,Mouse,Monkey |
| ICC | 技术咨询 | Human,Mouse,Monkey |
| FCM | 咨询技术 | Human,Mouse,Monkey |
| Elisa | 1/10000 | Human,Mouse,Monkey |
| Aliases | BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1 |
| Entrez GeneID | 4086 |
| clone | 4E12H10 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey |
| Immunogen | Purified recombinant fragment of human SMAD1 (AA: 1-110) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SMAD1抗体的3篇参考文献，涵盖其功能、应用及验证：
1. **"Regulation of Smad1 Activation in BMP Signaling"**
- **作者**: Hoodless, P.A. et al.
- **摘要**: 该研究解析了BMP信号中SMAD1的磷酸化机制及核转位过程。通过使用特异性SMAD1抗体，作者在成骨细胞模型中证实了SMAD1的C末端磷酸化对其转录活性的必要性，并揭示了其与SMAD4的协同作用。
2. **"Smad1 Signaling in Embryonic Stem Cell Differentiation"**
- **作者**: Liu, Y. et al.
- **摘要**: 本文利用SMAD1抗体通过免疫荧光和ChIP-seq技术，揭示了SMAD1在小鼠胚胎干细胞向中胚层分化中的关键作用。研究发现，SMAD1与特定靶基因启动子结合，调控多能性因子的表达。
3. **"Validation of a Phospho-Specific Smad1 Antibody in Vascular Development Studies"**
- **作者**: Upton, P.D. et al.
- **摘要**: 该文献详细验证了一种磷酸化SMAD1(Ser463/465)抗体的特异性，通过CRISPR敲除细胞系证明其信号依赖性。应用该抗体发现，BMP9激活的SMAD1磷酸化在血管内皮细胞成熟中起关键作用。
4. **"Aberrant Smad1 Expression in Colorectal Cancer Progression"**
- **作者**: Zhang, J. et al.
- **摘要**: 研究通过免疫组化及Western blot分析，使用SMAD1抗体证实其在结直肠癌组织中高表达，并与转移相关。机制研究表明，SMAD1通过上调EMT相关基因促进肿瘤侵袭。
这些文献覆盖了SMAD1抗体的功能研究、技术验证及疾病关联，适用于信号通路、发育和癌症领域的研究参考。
SMAD1 antibodies are essential tools for studying the SMAD1 protein, a key mediator of the bone morphogenetic protein (BMP) signaling pathway. SMAD1 belongs to the receptor-regulated SMAD (R-SMAD) family and is activated through phosphorylation by BMP type I receptors. Once activated, it forms complexes with SMAD4 (a co-SMAD) and translocates to the nucleus to regulate the transcription of target genes involved in cell differentiation, apoptosis, and embryonic development. SMAD1 plays critical roles in early embryogenesis, skeletal formation, and tissue homeostasis.
Antibodies targeting SMAD1 are widely used in biomedical research to detect protein expression, localization, and activation status in various biological samples. These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specific antibodies may distinguish between phosphorylated (active) and unphosphorylated (inactive) forms of SMAD1. providing insights into BMP pathway activity. Dysregulation of SMAD1 has been implicated in cancers, vascular disorders, and developmental defects, making these antibodies valuable for studying disease mechanisms. Researchers often validate SMAD1 antibodies using knockout cell lines or tissues to ensure specificity. Commercial antibodies are typically raised in rabbits or mice against epitopes within the N-terminal, linker, or C-terminal regions of SMAD1. with applications spanning basic research, diagnostics, and drug development.
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