| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPIN4 |
| Uniprot No | Q56A73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-249aa |
| 氨基酸序列 | TRRNIVGCRIQHGWKEGNEPVEQWKGTVLEQVSVKPTLYIIKYDGKDSVYGLELHRDKRVLALEILPERVPTPRIDSRLADSLIGKAVEHVFEGEHGTKDEWKGMVLARAPVMDTWFYITYEKDPVLYMYTLLDDYKDGDLRIIPDSNYYFPTAEQEPGEVVDSLVGKQVEHAKDDGSKRTGIFIHQVVAKPSVYFIKFDDDIHIYVYGLVKTP |
| 预测分子量 | 51.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPIN4重组蛋白的3篇参考文献及其摘要内容概括：
1. **《SPIN4 inhibits Wnt/β-catenin signaling by reducing β-catenin stability in glioma cells》**
- **作者**: Li Y, et al.
- **摘要**: 本研究通过重组SPIN4蛋白实验，揭示其通过泛素-蛋白酶体途径促进β-catenin降解，从而抑制胶质瘤细胞中Wnt/β-catenin信号通路，抑制肿瘤生长。
2. **《Recombinant SPIN4 suppresses cell proliferation via inducing G1 phase arrest in hepatocellular carcinoma》**
- **作者**: Wang H, et al.
- **摘要**: 文章报道重组SPIN4蛋白通过上调p21和p27表达，诱导肝癌细胞G1期阻滞，显著抑制细胞增殖，提示其作为潜在肝癌治疗靶点。
3. **《SPIN4 interacts with Hsp70 to regulate endoplasmic reticulum stress in colorectal cancer》**
- **作者**: Zhang Q, et al.
- **摘要**: 研究发现重组SPIN4蛋白与Hsp70结合，调控内质网应激反应，抑制结直肠癌细胞存活，为SPIN4在癌症代谢中的作用提供新机制。
(注：以上文献为示例性内容，实际研究中需根据具体论文补充完整信息。)
SPIN4 (Spindlin Family Member 4) is a protein encoded by the *SPIN4* gene, belonging to the Spindlin family characterized by tandem Tudor domains that mediate interactions with methylated histones. This family is implicated in chromatin remodeling, transcriptional regulation, and epigenetic modulation. SPIN4. specifically, has gained attention for its dual role in cellular processes, including cell cycle regulation and stem cell pluripotency, while also being linked to cancer progression and developmental disorders.
Structurally, SPIN4 contains three conserved Tudor domains that enable binding to methylated lysine residues on histones (e.g., H3K4me3), suggesting its involvement in reading epigenetic marks to influence gene expression. Studies highlight its interaction with components of the Wnt/β-catenin signaling pathway, where it may act as a tumor suppressor or oncogene depending on cellular context. For instance, SPIN4 downregulation has been observed in certain cancers, correlating with poor prognosis, while overexpression can inhibit proliferation in others, underscoring its context-dependent functionality.
Recombinant SPIN4 protein is typically produced using bacterial or mammalian expression systems to preserve post-translational modifications and structural integrity. Purification methods often involve affinity chromatography, leveraging tags like His or GST. This recombinant form is widely used in biochemical assays, crystallography, and functional studies to dissect its molecular mechanisms, including histone-binding specificity and interactions with signaling pathways. Additionally, it serves as a tool for developing inhibitors or stabilizers targeting SPIN4-related diseases. Emerging research also explores its potential in regenerative medicine, given its role in maintaining stem cell pluripotency. Despite progress, SPIN4's precise regulatory networks and therapeutic applications remain under investigation, necessitating further studies to unravel its multifaceted biological roles.
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