| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Fos |
| Uniprot No | P01100 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNAQDFCTDLAVSSANFIPTVTAISTSPDLQWLVQPALVSSVAPSQTRAPHPFGVPAPSAGAYSRAGVVKTMTGGRAQSIGRRGKVEQLSPEEEEKRRIRRERNKMAAAKCRNRRRELTDTLQAETDQLEDEKSALQTEIANLLKEKEKLEFILAAHRPACKIPDDLGFPEEMSVASLDLTGGLPEVATPESEEAFTLPLLNDPEPKPSVEPVKSISSMELKTEPFDDFLFPASSRPSGSETARSVPDMDLSGSFYAADWEPLHSGSLGMGPMATELEPLCTPVVTCTPSCTAYTSSFVFTYPEADSFPSCAAAHRKGSSSNEPSSDSLSSPTLLAL |
| 预测分子量 | 45.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant c-Fos protein: DNA binding and transcriptional activity"** by T. Curran, N. Teich (1982)
摘要：早期研究利用大肠杆菌表达重组c-Fos蛋白，证实其与Jun蛋白形成AP-1复合物，增强DNA结合能力并调控靶基因转录活性。
2. **"Purification of recombinant Fos protein and analysis of its interaction with Jun/AP-1 sites"** by L.C. Sambucetti, T. Curran (1986)
摘要：通过重组技术纯化v-Fos蛋白，发现其单独无法结合DNA，需与Jun家族蛋白形成异源二聚体以特异性识别AP-1启动子元件。
3. **"Fos-Jun heterodimers bind regulatory elements of stress-responsive genes"** by C. Abate et al. (1990)
摘要：研究重组Fos-Jun复合物与抗氧化反应元件(ARE)的结合特性，揭示其在氧化应激信号通路中调控基因表达的作用机制。
4. **"Structural studies of AP-1 transcription factor using recombinant Fos/Jun proteins"** by M. Karin et al. (1997)
摘要：通过X射线晶体学解析重组Fos/Jun蛋白二聚体结构，阐明其亮氨酸拉链结构域介导的相互作用及DNA结合模式，为癌症相关突变研究提供依据。
Fos recombinant proteins are derived from the Fos family of transcription factors, which play critical roles in regulating gene expression and cellular processes. The Fos family, including c-Fos, FosB, Fra-1. and Fra-2. forms heterodimers with Jun proteins (e.g., c-Jun) through leucine zipper interactions to create the activator protein-1 (AP-1) transcription factor complex. AP-1 binds to specific DNA sequences to regulate genes involved in cell proliferation, differentiation, apoptosis, and responses to stress or cytokines. Dysregulation of Fos proteins is implicated in cancer, inflammation, and developmental disorders.
Recombinant Fos proteins are engineered using genetic cloning techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. These proteins retain functional domains, such as the basic region for DNA binding and the leucine zipper for dimerization, enabling their use in studying AP-1-mediated signaling pathways. Researchers employ Fos recombinant proteins to investigate protein-DNA interactions, signal transduction mechanisms, and transcriptional regulation in vitro or in cellular models. They are also utilized in drug discovery to screen for inhibitors targeting AP-1 activity, which may have therapeutic potential in diseases like cancer or autoimmune disorders. Additionally, Fos recombinant proteins serve as tools in structural biology (e.g., crystallography) to elucidate molecular interactions within the AP-1 complex. Their versatility and specificity make them essential for dissecting the roles of Fos family members in health and disease.
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