| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HUWE1 |
| Uniprot No | Q7Z6Z7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4005-4374aa |
| 氨基酸序列 | LERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA |
| 预测分子量 | 50.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HUWE1重组蛋白的3篇参考文献，简要列举如下：
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1. **文献名称**：*HUWE1 E3 ligase promotes replication fork stall and recovery in BRCA1/2-deficient cells*
**作者**：Tarsounas M, et al.
**摘要**：该研究揭示了HUWE1作为E3泛素连接酶，在BRCA1/2缺陷的肿瘤细胞中调控复制叉稳定性。通过重组HUWE1蛋白的体外实验，证实其通过泛素化修饰MRE11核酸酶，促进DNA损伤修复的协调过程。
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2. **文献名称**：*Structural basis for HUWE1 recognition of substrate proteins and its role in proteasomal degradation*
**作者**：Zhang X, et al.
**摘要**：本文解析了HUWE1重组蛋白的晶体结构，揭示了其底物结合域(HECT结构域)的构象变化机制，并证明其通过动态泛素化调控p53、Myc等肿瘤相关蛋白的稳定性，影响细胞增殖与凋亡。
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3. **文献名称**：*HUWE1 regulates mitochondrial dynamics by promoting Mfn1 ubiquitination and degradation*
**作者**：Leboucher GP, et al.
**摘要**：研究利用重组HUWE1蛋白及敲除模型，发现其通过泛素化线粒体融合蛋白Mfn1.调控线粒体分裂与融合平衡，为神经退行性疾病中线粒体功能障碍的机制提供了新见解。
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如需更多文献或具体领域扩展，可进一步补充。
HUWE1 (HECT, UBA, and WWE domain-containing E3 ubiquitin protein ligase 1) is a multifunctional E3 ubiquitin ligase involved in regulating diverse cellular processes, including DNA repair, apoptosis, cell cycle progression, and mitochondrial homeostasis. It catalyzes the transfer of ubiquitin to target proteins, marking them for proteasomal degradation or modulating their activity. HUWE1 is notable for its broad substrate specificity, targeting key regulators such as p53. Myc, Mcl-1. and CDC6. which links it to cancer development, neurodevelopmental disorders, and aging-related pathologies. Its complex domain architecture, featuring a HECT catalytic domain, UBA domain, and WWE motifs, enables interactions with co-factors and substrates, though its regulatory mechanisms remain incompletely understood.
Recombinant HUWE1 protein is engineered for in vitro studies to dissect its enzymatic activity, substrate recognition, and structural properties. Produced using expression systems like bacteria, insect, or mammalian cells, the purified protein often includes tags (e.g., His-tag or GST) for affinity chromatography. Researchers employ it in ubiquitination assays, protein interaction mapping, and high-throughput drug screening to identify inhibitors for therapeutic applications. Structural studies using recombinant HUWE1 have shed light on conformational changes during catalysis and substrate binding. Mutant variants (e.g., catalytic cysteine mutants) are also generated to study loss-of-function mechanisms or disease-associated mutations. Despite challenges in maintaining its stability due to its large size (~480 kDa) and multi-domain flexibility, recombinant HUWE1 remains a critical tool for unraveling its roles in cellular signaling and disease pathways.
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