| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPYSL4 |
| Uniprot No | O14531 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 280-560aa |
| 氨基酸序列 | TASLGTDGSHYWSKNWAKAAAFVTSPPVNPDPTTADHLTCLLSSGDLQVTGSAHCTFTTAQKAVGKDNFALIPEGTNGIEERMSMVWEKCVASGKMDENEFVAVTSTNAAKIFNFYPRKGRVAVGSDADLVIWNPKATKIISAKTHNLNVEYNIFEGVECRGAPAVVISQGRVALEDGKMFVTPGAGRFVPRKTFPDFVYKRIKARNRLAEIHGVPRGVYDGPVHEVMVPAKPGSGAPARASCPGKISVPPVRNLHQSGFSLSGSQADDHIARRTAQKIMA |
| 预测分子量 | 66.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPYSL4重组蛋白的3篇模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **标题**: *DPYSL4 Recombinant Protein Modulates Axon Guidance via Semaphorin Signaling*
**作者**: Chen L, et al.
**摘要**: 研究利用大肠杆菌表达系统制备重组DPYSL4蛋白，证实其通过结合Semaphorin 3A调控神经元轴突导向，并解析了其C端结构域在信号传导中的关键作用。
2. **标题**: *CRMP4/DPYSL4 Recombinant Protein as a Metastasis Biomarker in Colorectal Cancer*
**作者**: Tanaka K, et al.
**摘要**: 通过哺乳动物细胞表达纯化DPYSL4重组蛋白，发现其与结直肠癌转移正相关，体外实验表明重组蛋白可增强癌细胞迁移能力，提示其作为治疗靶点的潜力。
3. **标题**: *Structural Characterization of DPYSL4 Recombinant Protein and Its Interaction with Tubulin*
**作者**: Müller S, et al.
**摘要**: 采用杆状病毒系统表达DPYSL4重组蛋白，通过X射线晶体学解析三维结构，揭示其与微管蛋白结合的活性位点，为神经退行性疾病药物设计提供依据。
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**提示**：实际文献检索建议使用PubMed、Web of Science等平台，以“DPYSL4 recombinant protein”或“CRMP4 expression”为关键词，并筛选涉及蛋白表达、功能或应用的文献。
**Background of DPYSL4 Recombinant Protein**
DPYSL4 (Dihydropyrimidinase-like 4), also known as CRMP-3 (Collapsin Response Mediator Protein 3), is a cytosolic phosphoprotein belonging to the dihydropyrimidinase-related protein family. It plays critical roles in neurodevelopment, including neuronal differentiation, axon guidance, and growth cone collapse, by modulating cytoskeletal dynamics and intracellular signaling pathways. DPYSL4 interacts with tubulin heterodimers and other cytoskeletal components, influencing microtubule assembly and stability, which is essential for neurite outgrowth and synaptic plasticity.
The recombinant DPYSL4 protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research applications. Its production often involves affinity tags (e.g., His-tag) for simplified purification. Recombinant DPYSL4 serves as a vital tool for studying neurological disorders, such as Alzheimer’s disease and schizophrenia, where its expression is dysregulated. It also contributes to cancer research, as DPYSL4 is implicated in tumor metastasis via pathways linked to epithelial-mesenchymal transition (EMT) and cell migration.
Structurally, DPYSL4 contains conserved domains for binding to cytoskeletal elements and signaling molecules like Rho GTPases, making it a focal point for investigating cellular motility and morphology. Researchers utilize this recombinant protein in *in vitro* assays, drug discovery, and mechanistic studies to unravel its role in health and disease. Its versatility underscores its importance in both basic neuroscience and translational oncology.
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