| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDDC2 |
| Uniprot No | Q7Z4H3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMASVSSA TFSGHGARSL LQFLRLVGQL KRVPRTGWVY RNVQRPESVS DHMYRMAVMA MVIKDDRLNK DRCVRLALVH DMAECIVGDI APADNIPKEE KHRREEEAMK QITQLLPEDL RKELYELWEE YETQSSAEAK FVKQLDQCEM ILQASEYEDL EHKPGRLQDF YDSTAGKFNH PEIVQLVSEL EAERSTNIAA AASEPHS |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDDC2重组蛋白的假设性参考文献示例(请注意，实际文献可能需要通过学术数据库验证或调整关键词搜索)：
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1. **文献名称**: "Cloning and Functional Characterization of HDDC2 Recombinant Protein in Hepatocyte Differentiation"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了HDDC2基因的克隆及重组蛋白在大肠杆菌中的表达与纯化。实验表明，HDDC2重组蛋白通过调控Wnt/β-catenin信号通路促进肝细胞分化，可能为肝脏再生治疗提供新靶点。
2. **文献名称**: "HDDC2 Recombinant Protein Attenuates Inflammatory Response in Macrophages via NF-κB Inhibition"
**作者**: Kim S, et al.
**摘要**: 通过哺乳动物细胞系统表达HDDC2重组蛋白，研究发现其能结合TRAF6并抑制NF-κB活化，显著降低LPS诱导的巨噬细胞炎症因子释放，提示其在抗炎治疗中的潜在应用。
3. **文献名称**: "Structural Analysis of HDDC2 Recombinant Protein and Its Role in DNA Damage Repair"
**作者**: Müller R, et al.
**摘要**: 利用X射线晶体学解析了HDDC2重组蛋白的三维结构，发现其C端结构域与DNA损伤修复相关。功能实验证实HDDC2通过增强ATM激酶活性参与DNA双链断裂修复。
4. **文献名称**: "Expression Optimization and Functional Screening of HDDC2 Recombinant Protein in Cancer Cell Lines"
**作者**: Gupta P, et al.
**摘要**: 优化了HDDC2在昆虫杆状病毒系统中的表达条件，发现其重组蛋白可诱导结肠癌细胞凋亡，机制可能与抑制PI3K/AKT通路及上调促凋亡蛋白Bax有关。
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**注意**：以上内容为示例性模拟，实际文献需通过PubMed、Google Scholar等平台以关键词“HDDC2 recombinant protein”“HDDC2 gene function”等检索。若搜索结果有限，建议核查拼写(如是否应为HDAC2、HGDAC2等类似名称)或扩展相关领域关键词。
HDDC2 (Histone Deacetylase Domain-Containing Protein 2), also known as HDAC11-like protein, is a member of the histone deacetylase (HDAC) family, which plays a critical role in epigenetic regulation by modifying chromatin structure and gene expression. Unlike classical HDACs, HDDC2 contains a conserved deacetylase domain but exhibits distinct structural and functional characteristics. It is implicated in diverse cellular processes, including transcriptional repression, cell cycle regulation, and metabolic homeostasis. Dysregulation of HDDC2 has been associated with various diseases, such as cancer, neurodegenerative disorders, and immune dysfunction, making it a potential therapeutic target.
Recombinant HDDC2 protein is engineered using biotechnological methods, often expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. The recombinant form typically retains enzymatic activity, enabling researchers to study its biochemical properties, substrate specificity, and interactions with inhibitors or cofactors. Purification techniques, such as affinity chromatography with tags like His or GST, ensure high purity and stability for experimental use.
Research on recombinant HDDC2 focuses on elucidating its role in epigenetic mechanisms and disease pathways. It is widely used in *in vitro* assays to screen for HDAC inhibitors, investigate enzyme kinetics, and explore its regulatory effects on histone and non-histone substrates. Additionally, structural studies leveraging recombinant HDDC2 contribute to understanding its catalytic mechanism and designing targeted therapies. As interest in epigenetic therapeutics grows, HDDC2 remains a key protein for advancing drug discovery and mechanistic studies in molecular biology.
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