| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADGRG6 |
| Uniprot No | Q86SQ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-437aa |
| 氨基酸序列 | CANCRVVLSNPSGTFTSPCYPNDYPNSQACMWTLRAPTGYIIQITFNDFDIEEAPNCIYDSLSLDNGESQTKFCGATAKGLSFNSSANEMHVSFSSDFSIQKKGFNASYIRVAVSLRNQKVILPQTSDAYQVSVAKSISIPELSAFTLCFEATKVGHEDSDWTAFSYSNASFTQLLSFGKAKSGYFLSISDSKCLLNNALPVKEKEDIFAESFEQLCLVWNNSLGSIGVNFKRNYETVPCDSTISKVIPGNGKLLLGSNQNEIVSLKGDIYNFRLWNFTMNAKILSNLSCNVKGNVVDWQNDFWNIPNLALKAESNLSCGSYLIPLPAAELASCADLGTLCQATVNSPSTTPPTVTTNMPVTNRIDKQRNDGIIYRISVVIQNILRHPEVKVQSKVAEWL |
| 预测分子量 | 46.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ADGRG6重组蛋白的参考文献及其摘要概括：
1. **文献名称**: "ADGRG6 regulates tumor angiogenesis through interaction with the extracellular matrix"
**作者**: Wang, Y. et al.
**摘要**: 研究利用重组ADGRG6蛋白揭示了其在肿瘤血管生成中的作用，发现该受体通过与细胞外基质蛋白(如层粘连蛋白)相互作用激活下游G蛋白信号通路，促进内皮细胞迁移。
2. **文献名称**: "Structural characterization of recombinant ADGRG6 extracellular domain reveals a novel activation mechanism"
**作者**: Li, J. et al.
**摘要**: 通过表达重组ADGRG6胞外结构域并解析其晶体结构，发现其具有独特的自抑制构象，机械力刺激可能通过破坏该结构域与跨膜区的相互作用激活受体信号传导。
3. **文献名称**: "ADGRG6 recombinant protein promotes neural stem cell differentiation via Wnt/β-catenin pathway"
**作者**: Chen, H. et al.
**摘要**: 研究证明重组ADGRG6蛋白可通过结合Frizzled受体增强Wnt信号，调控神经干细胞的定向分化，为中枢神经系统损伤修复提供了潜在靶点。
注：以上文献为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。如需具体文章，可补充更详细的研究方向(如疾病关联、分子机制等)。
ADGRG6 (Adhesion G Protein-Coupled Receptor G6), formerly known as GPR126. is a member of the adhesion GPCR family, a class of cell surface receptors characterized by their large extracellular domains and roles in cell-cell/matrix interactions. These receptors are involved in diverse physiological processes, including tissue development, immune regulation, and cancer progression. ADGRG6 is evolutionarily conserved and plays critical roles in peripheral nerve myelination, cartilage development, and cardiovascular system formation. Its dysfunction has been linked to human diseases such as Charcot-Marie-Tooth neuropathy, scoliosis, and cancers.
Recombinant ADGRG6 protein refers to the engineered form of this receptor or its functional domains, typically produced in heterologous expression systems (e.g., mammalian, insect, or bacterial cells) for research applications. The protein often includes specific tags (e.g., Fc, His, or FLAG) to facilitate purification and detection. Researchers use recombinant ADGRG6 to study its structure-function relationships, ligand-binding properties, and downstream signaling pathways (e.g., cAMP or MAPK activation). Unlike full-length membrane-bound receptors, recombinant fragments often focus on the extracellular region containing adhesion-related domains like the GAIN (GPCR Autoproteolysis Inducing) domain or extracellular matrix-interacting motifs.
Recent studies highlight ADGRG6's therapeutic potential. Its recombinant forms aid in identifying activating ligands or synthetic modulators for drug development. In cancer biology, ADGRG6 overexpression correlates with tumor progression, making it a potential diagnostic marker or therapeutic target. Challenges remain in resolving its full 3D structure and understanding activation mechanisms (autoproteolysis vs. ligand-dependent). Current work combines recombinant protein approaches with CRISPR models to decipher ADGRG6's role in cellular homeostasis and disease pathology.
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