| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BCAM; BCT2; PP18; BCATM |
| Entrez GeneID | 587 |
| clone | 7G3A11 |
| WB Predicted band size | 44.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BCAT2 (AA: 259-393) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于BCAT2抗体的3篇参考文献示例(注：文献为假设性示例，实际引用需核实真实来源)：
1. **文献名称**：*BCAT2 regulates glutamine metabolism and promotes cancer cell proliferation in glioblastoma*
**作者**：Zhang Y, et al.
**摘要**：本研究通过Western blot和免疫荧光技术(使用BCAT2特异性抗体)揭示了BCAT2在胶质母细胞瘤细胞中对谷氨酰胺代谢的调控作用，其高表达与肿瘤细胞增殖和患者预后不良相关。
2. **文献名称**：*A validated BCAT2 monoclonal antibody reveals tissue-specific expression patterns in murine models*
**作者**：Thompson R, et al.
**摘要**：报道了一种经验证的高特异性BCAT2单克隆抗体的开发，并通过免疫组化分析展示了BCAT2在小鼠肝脏、肌肉及脑组织中的差异表达，为代谢研究提供可靠工具。
3. **文献名称**：*BCAT2-mediated branched-chain amino acid catabolism links obesity to metabolic dysfunction*
**作者**：Chen L, et al.
**摘要**：利用BCAT2抗体进行蛋白质印迹和免疫沉淀实验，发现肥胖模型中BCAT2活性升高导致支链氨基酸异常代谢，进而加剧胰岛素抵抗和脂肪肝病变。
(提示：实际研究中建议通过PubMed或Web of Science以“BCAT2 antibody”为关键词检索最新文献。)
**Background of BCAT2 Antibody**
The Branched-Chain Amino Acid Transaminase 2 (BCAT2) is a mitochondrial enzyme that catalyzes the reversible transamination of branched-chain amino acids (BCAAs: leucine, isoleucine, valine) into their corresponding α-keto acids, playing a critical role in nitrogen metabolism and energy production. BCAT2 is highly expressed in tissues with active mitochondrial metabolism, such as the liver, kidney, and brain, and is implicated in regulating cellular processes like growth, nutrient signaling, and redox balance.
BCAT2 antibodies are essential tools for studying the enzyme's expression, localization, and function in physiological and pathological contexts. Dysregulation of BCAT2 has been linked to metabolic disorders, neurodegenerative diseases, and cancer. For example, elevated BCAT2 levels in certain cancers are associated with enhanced BCAA catabolism, promoting tumor growth and chemoresistance.
These antibodies enable detection of BCAT2 via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Validated antibodies are critical for specificity, as BCAT2 shares homology with its cytosolic isoform, BCAT1. Research applications include exploring BCAT2's role in metabolic reprogramming, mitochondrial dysfunction, and disease mechanisms. Commercial BCAT2 antibodies are typically raised against peptide sequences unique to the human or murine protein, with validation in knockout models or siRNA-treated samples to confirm target specificity.
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