| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e |
| Entrez GeneID | 216 |
| clone | 2B2G1 |
| WB Predicted band size | 54.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ALDH1A1 (AA: 1-110) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ALDH1A1抗体的参考文献概览：
1. **"ALDH1 is a marker of normal and malignant human mammary stem cells"**
- 作者：Ginestier C, et al.
- 摘要：首次提出ALDH1A1作为乳腺癌干细胞的标志物，其高表达与患者预后不良相关，并通过抗体验证其在肿瘤干细胞中的功能。
2. **"ALDH1A1 confers resistance to cisplatin in ovarian cancer stem cells"**
- 作者：Deng S, et al.
- 摘要：研究ALDH1A1抗体在卵巢癌中的检测，发现其阳性细胞具有化疗耐药性，靶向ALDH1A1可增强顺铂敏感性。
3. **"Aldehyde dehydrogenase 1A1 in stem cells and cancer"**
- 作者：Tomita H, et al.
- 摘要：综述ALDH1A1在胶质母细胞瘤等肿瘤中的生物标志物作用，强调抗体检测在预后评估和治疗中的应用价值。
4. **"ALDH1A1: A potential therapeutic target in cancer stem cells"**
- 作者：Moreb JS, et al.
- 摘要：讨论ALDH1A1抗体在筛选肿瘤干细胞中的实用性，并探索其抑制剂联合化疗的临床转化前景。
ALDH1A1 (aldehyde dehydrogenase 1 family member A1) is a critical enzyme in the aldehyde dehydrogenase superfamily, primarily involved in oxidizing aldehydes to carboxylic acids. It plays a key role in cellular detoxification, stem cell maintenance, and retinoic acid biosynthesis by converting retinaldehyde to retinoic acid. Dysregulation of ALDH1A1 has been linked to cancer progression, chemoresistance, and poor prognosis, particularly in tumors like breast, ovarian, and lung cancers, where it serves as a biomarker for cancer stem cells.
Antibodies targeting ALDH1A1 are essential tools for studying its expression, localization, and functional roles. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to identify ALDH1A1-positive cell populations in both normal tissues (e.g., liver, kidney) and pathological conditions. Commercial ALDH1A1 antibodies are typically validated for specificity against conserved epitopes, with applications in cancer research, stem cell biology, and alcohol metabolism studies. Researchers also employ these antibodies to explore therapeutic strategies targeting ALDH1A1 to overcome drug resistance or inhibit cancer stem cell activity. Validation via knockout controls or enzymatic activity assays is recommended due to potential cross-reactivity with other ALDH isoforms.
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