| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | cia |
| Uniprot No | P06716 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626aa |
| 氨基酸序列 | MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPPAWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRLSAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHTESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNYLLSHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAESAVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLSGINQKIAEEKRKQDELKATKDAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKINAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRPLFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFWGI |
| 预测分子量 | 76.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CIA重组蛋白的模拟参考文献示例(注：文献为虚构，仅供格式参考)：
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1. **文献名称**：*Heterologous Expression and Functional Analysis of the CIA Recombinant Protein Complex in Saccharomyces cerevisiae*
**作者**：Miller R, et al.
**摘要**：本研究利用酵母系统成功表达并纯化了重组CIA复合体(CIAO1、CIA2A、MMS19)，通过遗传互补实验证实其在胞质铁硫蛋白成熟中的关键作用，并揭示其与线粒体铁硫转运的协同机制。
2. **文献名称**：*Cryo-EM Structure of the Human CIA2B-MMS19 Recombinant Protein Complex*
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析人源CIA2B与MMS19重组蛋白复合体的高分辨率结构，阐明两者结合界面及底物识别位点，为铁硫簇传递的分子机制提供结构基础。
3. **文献名称**：*In Vitro Reconstitution of the Cytosolic Iron-Sulfur Cluster Assembly Pathway Using Recombinant CIA Proteins*
**作者**：Johnson T, et al.
**摘要**：开发了一种基于重组CIA蛋白(CIAO1、CIA2B)的体外铁硫簇组装体系，证明其能将铁硫簇转移至靶蛋白apo-aconitase，并量化其催化效率。
4. **文献名称**：*Functional Interaction Between Recombinant CIA Proteins and DNA Helicases in Genome Maintenance*
**作者**：Wang Q, et al.
**摘要**：通过共表达重组CIA复合体与DNA解旋酶(如XPD)，发现CIA蛋白通过铁硫簇依赖的机制调控DNA修复酶的活性，影响基因组稳定性。
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**说明**：以上文献及摘要均为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索关键词“CIA complex recombinant”、“cytosolic iron-sulfur cluster assembly”等获取真实文献。
**Background of CIA Recombinant Proteins**
The Cytosolic Iron-sulfur protein Assembly (CIA) pathway is a conserved biological system responsible for synthesizing and inserting iron-sulfur (Fe-S) clusters into cytosolic and nuclear proteins. Fe-S clusters are ancient cofactors critical for enzymes involved in DNA repair, ribosome biogenesis, and metabolic pathways. While mitochondrial systems handle Fe-S assembly for mitochondrial proteins, the CIA pathway specifically serves extramitochondrial proteins, making it essential for eukaryotic cell viability.
Recombinant CIA proteins are engineered versions of key components in this pathway, such as CIA1 (CIAO1), CIA2A/B, and MMS19. These proteins are produced using genetic engineering techniques (e.g., bacterial or eukaryotic expression systems) to study their structure, function, and interactions. Recombinant expression allows large-scale production, enabling biochemical assays, structural studies (e.g., X-ray crystallography), and functional analyses in vitro or in cellular models.
Research on CIA recombinant proteins has clarified their roles in Fe-S cluster transfer. For instance, CIA2A interacts with nascent Fe-S recipient proteins, while CIA1 acts as a scaffold. Dysregulation of the CIA pathway is linked to diseases, including cancers and neurodegenerative disorders, as Fe-S-dependent enzymes are vital for genome stability and redox homeostasis. Recombinant CIA proteins thus serve as tools to explore disease mechanisms or screen for therapeutic agents targeting Fe-S metabolism.
Additionally, these proteins aid in biotechnology applications, such as engineering Fe-S enzymes for industrial catalysis or synthetic biology. By unraveling CIA pathway mechanics, recombinant proteins contribute to both basic science and translational innovations.
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