| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGF2BP1 |
| Uniprot No | Q9NZI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-577aa |
| 氨基酸序列 | MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGKVELQGKRLEIEHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETAVVNVTYSNREQTRQAIMKLNGHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQGSPVAAGAPAKQQQVDIPLRLLVPTQYVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLKILAHNNFVGRLIGKEGRNLKKVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREAYENDVAAMSLQSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAIIGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLETHIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKIIGHFYASQMAQRKIRDILAQVKQQHQKGQSNQAQARRK |
| 预测分子量 | 69.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于IGF2BP1重组蛋白的相关文献信息(注：文献为虚拟概括，实际引用请以真实论文为准)：
1. **标题**: "Recombinant IGF2BP1 promotes cancer cell adhesion by stabilizing β-catenin mRNA"
**作者**: Smith A, et al.
**摘要**: 研究利用重组IGF2BP1蛋白验证其通过结合β-catenin mRNA的3'UTR增强稳定性，促进肿瘤细胞粘附和侵袭的分子机制。
2. **标题**: "Expression and purification of human IGF2BP1 for RNA-binding assays"
**作者**: Chen L, et al.
**摘要**: 描述了在大肠杆菌系统中高效表达并纯化重组人源IGF2BP1蛋白的方法，验证其与靶标RNA的结合活性，为体外功能研究提供工具。
3. **标题**: "IGF2BP1 recombinant protein rescues neuronal development deficits in cellular models"
**作者**: Wang Y, et al.
**摘要**: 通过添加重组IGF2BP1蛋白，证明其可调控神经突触相关mRNA的翻译，改善神经细胞分化障碍，提示其在神经疾病中的潜在治疗价值。
4. **标题**: "Structural analysis of IGF2BP1 KH domains by recombinant protein crystallography"
**作者**: Gonzalez R, et al.
**摘要**: 利用重组IGF2BP1蛋白的KH结构域进行晶体结构解析，揭示其特异性识别RNA序列的结构基础，为靶向药物设计提供依据。
(注：以上文献为示例性质，实际文献需通过PubMed/Google Scholar检索确认。)
**Background of IGF2BP1 Recombinant Protein**
IGF2BP1 (Insulin-like Growth Factor 2 mRNA-Binding Protein 1) is a member of the conserved RNA-binding protein family, which includes IGF2BP1-3. It plays critical roles in post-transcriptional gene regulation by stabilizing target mRNAs, enhancing their translation, and influencing subcellular localization. Primarily expressed during embryonic development, IGF2BP1 is often re-expressed in cancers, where it promotes tumorigenesis by regulating oncogenic mRNAs (e.g., MYC, ACTB) linked to proliferation, invasion, and metastasis. Its dysregulation is associated with poor prognosis in multiple malignancies.
Recombinant IGF2BP1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce a purified, functional form for *in vitro* studies. This recombinant tool retains key structural domains, such as the RNA-binding K-homology (KH) domains and a regulatory region, enabling investigations into its RNA-binding mechanisms and interactions. Researchers employ it to dissect IGF2BP1's role in RNA metabolism, its interplay with signaling pathways (e.g., Wnt/β-catenin), and its contribution to cancer progression.
Additionally, recombinant IGF2BP1 aids in drug discovery, serving as a target for screening inhibitors that disrupt its oncogenic RNA interactions. Its applications extend to developmental biology, where it helps elucidate mRNA regulation during embryogenesis. By providing a controlled, scalable protein source, recombinant IGF2BP1 accelerates both basic research and therapeutic development, offering insights into its dual roles as a developmental regulator and a cancer-associated antigen.
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